Skin samples containing the wound edge and newly formed dermal tissue were collected from patient 2 in the third postoperative week, and the specimen was immersed in 10% formalin. The sample was dehydrated using an ethanol gradient and embedded in paraffin. Micrometre‐level thin tissue slices were prepared, and Haematoxylin & Eosin, Masson's trichrome staining were performed. Photomicrographs were collected using a Leica DFC295 microscope.
Dfc295 microscope
Manufactured by Leica
Sourced in Germany
The Leica DFC295 is a digital microscope camera that captures high-quality digital images from microscope specimens. It features a 2.8-megapixel CMOS sensor and supports various resolutions for imaging. The camera can be connected to a computer for image acquisition and processing.
Automatically generated - may contain errors
Lab products found in correlation
Axiovert 200m inverted microscope, by Zeiss (1 mentions)
Axiovision acquisition software, by Zeiss (1 mentions)
Goat anti mouse igg2a fitc, by Southern Biotech (1 mentions)
Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions)
Application suite software, by Leica (1 mentions)
Crystal violet, by Solarbio (1 mentions)
Transwell chamber, by BD (1 mentions)
4 protocols using dfc295 microscope
1
Histological Analysis of Skin Wound Healing
2
Lung Tissue Immunofluorescence Analysis
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Lungs were fixed for 24 h with 10% formalin and were kept inflated at constant pressure throughout the fixation process. Lung sections were stained with hematoxylin and eosin, and were evaluated blindly by two experienced lung pathologists. Images were taken with a Leica DFC295 microscope. Immunofluorescence staining was performed on 4 μm acetone-fixed frozen lung and kidney sections. Tissue sections were incubated with the primary anti-α-SMA (Cat. Nr: 61001, ProGen, Heidelberg, Germany) antibody for 1 h at RT. Binding of α-SMA primary antibody was detected by incubation with goat anti-mouse IgG2a FITC (Southern Biotech, Birmingham, AL, USA) for 30 min. Nuclei were stained with Hoechst, and sections were coverslipped with Prolong Gold antifade reagent (Invitrogen). All immunofluorescence images were taken with a Zeiss Axiovert 200M inverted microscope and AxioVision acquisition software (Carl Zeiss, Oberkochen, Germany).
3
Prostate Tissue Histological Analysis
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Prostate tissues from each group were fixed in 4% formalin and embedded in paraffin. Tissues were cut into 4-μm sections. The sections were stained with hematoxylin and eosin (H&E) for histological examination. Images were acquired using a Leica DFC 295 microscope (Leica, Wetzlar, Germany). The thickness of the epithelium in the prostate tissue was measured using the Leica Application Suite software (LAS ver. 3.3.0; Leica Microsystems, Inc., Buffalo Grove, IL, USA).
4
Transwell Cell Invasion Assay Protocol
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The cell invasion assay was performed using the Transwell chamber (BD BioCoat, Shanghai, China). Briefly, 300 µL of H-DMEM incubated at 37 °C was added to each Transwell chamber and left at room temperature for 20 min. After the intervention, cells were seeded into 24-well polycarbonate Transwell chambers with 8-μm pores at 1.5 × 104 cells/well in 200 µL of serum-free medium, with the lower chamber being covered with 600 µL of the complete medium as a chemoattractant. After incubation for 32 h at 37 °C and 5% CO2, the noninvaded cells on the upper chamber were mechanically wiped off. The invaded cells were washed with PBS, immobilized in 4% paraformaldehyde for 5 min, dyed with 0.1% crystal violet for 20 min (Solarbio, Beijing, China), and quantified using six randomly selected fields of view under a Leica DFC295 microscope (Leica DFC295, Germany). Three independent experiments were conducted.
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