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Automated staining system

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The Leica automated staining system is a laboratory equipment designed for the automated processing of tissue samples. It automates the staining process, a critical step in histological analysis, to ensure consistent and standardized results. The system handles the application of reagents, incubation, and washing steps required for various staining protocols.

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Bond Max automated staining system (9 citations) Bond fully-automated slide staining system (4 citations) BOND-MAX Fully Automated IHC Staining System (2 citations) Automated slide staining system (2 citations) Bond automated staining system (2 citations) BOND-MAX Automated IHC Staining System (2 citations)

2 protocols using automated staining system

1

Histological Analysis of MERS-CoV Infection

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Forty-one tissues from all major organ systems were collected and fixed in 10% neutral buffered formalin (NBF) for 30 days. Following fixation in 10% NBF tissues were processed following standard procedures. Hematoxylin and eosin (HE) stain was applied using the Leica automated staining system (Leica, Microsystems). Stained slides were then examined via standard light microscopy. To detect MERS-CoV antigen, IHC was performed using a rabbit polyclonal antiserum against MERS-CoV (Sino Biological P.R. China) (1:1000). Tissues from an uninfected control animal were used to validate all IHC procedures. HE and IHC sections were examined by light microscopy by the veterinary pathologists (SY and LH).
Johnson R.F., Via L.E., Kumar M.R., Cornish J.P., Yellayi S., Huzella L., Postnikova E., Oberlander N., Bartos C., Ork B.L., Mazur S., Allan C., Holbrook M.R., Solomon J., Johnson J.C., Pickel J., Hensley L.E, & Jahrling P.B. (2015). Intratracheal exposure of common marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 isolates does not result in lethal disease. Virology, 485, 422-430.
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2

Tissue Collection and MERS-CoV Detection

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Forty-one tissues from all major organ systems were collected and fixed in 10% neutral buffered formalin (NBF) for 30 days. Following fixation in 10% NBF tissues were processed following standard procedures. Hematoxylin and eosin (HE) stain was applied using the Leica automated staining system (Leica, Microsystems). Stained slides were then examined via standard light microscopy. To detect MERS-CoV antigen, IHC was performed using a rabbit polyclonal antiserum against MERS-CoV (Sino Biological PR China) (1:1000). Tissues from an uninfected control animal were used to validate all IHC procedures. HE and IHC sections were examined by light microscopy by the veterinary pathologists (SY and LH).
Johnson R.F., Via L.E., Kumar M.R., Cornish J.P., Yellayi S., Huzella L., Postnikova E., Oberlander N., Bartos C., Ork B.L., Mazur S., Allan C., Holbrook M.R., Solomon J., Johnson J.C., Pickel J., Hensley L.E, & Jahrling P.B. (2015). Intratracheal exposure of common marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 isolates does not result in lethal disease. Virology, 485, 422-430.
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