The viral RNA of nasopharyngeal specimens was extracted with a Magnapure 96 instrument (Roche, Mannheim, Germany) using the DNA and Viral nucleic acid kit (Roche, Penzberg, Germany). The RNA concentration in each sample was determined by reverse transcriptase polymerase chain reaction (RT-PCR) using the LightMix® Modular SARS-CE assay (50-0776-96, TIB MolBiol, Berlin, Germany) and programming on 480II light cycler (Roche, Penzberg, Germany). The point at which the amplification curve crossed the vertical threshold line in the RT PCR cycle (Cp) was reported as positive if the sample Cp was less than 40.
Lightmix modular sars ce assay
Manufactured by TIB Molbiol
Sourced in Germany
The LightMix® Modular SARS-CE assay is a laboratory testing kit developed by TIB Molbiol. It is designed to detect the presence of the SARS-CoV-2 virus, which causes COVID-19. The assay utilizes real-time PCR technology to identify specific genetic sequences of the virus, providing users with information about the presence of the virus in a sample.
Automatically generated - may contain errors
3 protocols using lightmix modular sars ce assay
1
SARS-CoV-2 Detection from Nasopharyngeal Samples
2
SARS-CoV-2 RNA Detection in Nasopharyngeal Swabs
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The viral RNA of nasopharyngeal specimens was extracted with a Magnapure 96 instrument (Roche, Mannheim, Germany) using the DNA and Viral Nucleic Acid Kit (Roche, Penzberg, Germany). The RNA concentration in each sample was determined by reverse transcriptase polymerase chain reaction (RT-PCR) using the LightMix® Modular SARS-CE assay (40-0770 and 60-0770, TIB MolBiol, Berlin, Germany) and programming on a 480II light cycler (Roche, Penzberg, Germany). The point at which the amplification curve of the E gene crossed the vertical threshold line in the RT PCR cycle (Ct) was reported as positive if the sample Ct was less than 40.
3
SARS-CoV-2 Specimen Collection and Testing
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Nasopharyngeal specimens were collected and stored at −80 °C in a virus collection and preservation medium (Khang Jian Medical Apparatus Ltd., Taizhou, China) and transported on dry ice to Germany There were 3 cohorts: cohort 1 (n = 25), cohort 2 (n = 23), and cohort 3 (n = 78). Cohort 1 serum samples were collected between day 19 and day 27 post onset of COVID-19 symptoms (POCS), cohort 2 serum samples with BA.2 omicron variant were collected between day 186 and day 237 POCS. Cohort 3 serum samples were collected between day 33 and day 113 POCS. All samples were preserved in screw-tight cryopreservation vials (Greiner-bio) at a temperature of −20 °C.
The viral RNA of nasopharyngeal specimens was extracted with a Magnapure 96 instrument (Roche, Mannheim, Germany) using the DNA and viral nucleic acid Kit (Roche, Penzberg, Germany). The RNA concentration in each sample was determined by reverse transcriptase polymerase chain reaction (RT-PCR) using the LightMix® Modular SARS-CE assay (40–0770 and 60–0770, TIB MolBiol, Berlin, Germany) and programming on a 480II light cycler (Roche, Penzberg, Germany). The point at which the amplification curve of the E gene crossed the vertical threshold line in the RT PCR cycle (Ct) was reported as positive if the sample Ct was less than 40.
The viral RNA of nasopharyngeal specimens was extracted with a Magnapure 96 instrument (Roche, Mannheim, Germany) using the DNA and viral nucleic acid Kit (Roche, Penzberg, Germany). The RNA concentration in each sample was determined by reverse transcriptase polymerase chain reaction (RT-PCR) using the LightMix® Modular SARS-CE assay (40–0770 and 60–0770, TIB MolBiol, Berlin, Germany) and programming on a 480II light cycler (Roche, Penzberg, Germany). The point at which the amplification curve of the E gene crossed the vertical threshold line in the RT PCR cycle (Ct) was reported as positive if the sample Ct was less than 40.
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