Ag07095

Human foreskin fibroblasts from male patient with DS (DS) and male diploid age-matched controls (2N) were purchased from the Coriell Institute for Medical Research (2-year-old: AG06922 and AG07095; 5-month-old: AG07096 and GM08680) and maintained according to the distributor’s protocols (www.coriell.org/). Throughout this study, fibroblasts were used below passage number 15 to keep the original character and morphology.
siRNA against human APP and negative control DsiRNA were purchased from Integrated DNA Technologies (IDT) and used as previously described (31 (link)). siRNA against human Fyn was purchased from Life Technologies Corp. Cells were transfected using Lipofectamine RNAiMAX according to the manufacturer’s protocol.
pcDNA-APP-βCTF was previously described (31 (link)). Cells were transiently transfected using Lipofectamine 2000 according to the manufacturer’s protocol.

Human foreskin fibroblasts (AG07095) were purchased from Coriell Institute for Medical Research (Camden, N.J.). Passage 5 to 9 cells were grown in monolayer either on plastic culture dishes (Falcon, Lincoln Park, N.J.) or on sterile glass coverslips (Fisher, Itasca, Ill.). They were cultured in the Dulbecco modified Eagle medium (DMEM, Gibco, Grand Island, N.Y.) with 10% fetal bovine serum (FBS, Hyclone, Logan, Utah). Cell viability was high.