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Apotag plus peroxidase in situ tunel apoptosis kit

Manufactured by Merck Group
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The ApoTag Plus Peroxidase in situ TUNEL Apoptosis Kit is a laboratory equipment product designed for the detection and analysis of apoptosis, a type of programmed cell death. The kit utilizes the TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) assay, which labels the fragmented DNA that is characteristic of apoptosis. The kit includes reagents and buffers necessary for the TUNEL reaction and subsequent visualization of the labeled DNA fragments.

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Lab products found in correlation

Foxo1, by Thermo Fisher Scientific (2 mentions) P akt ser473, by Thermo Fisher Scientific (2 mentions) Ps6rp, by Cell Signaling Technology (2 mentions) P erk, by Cell Signaling Technology (2 mentions)

Spelling variants of this product

TUNEL kit (63 citations) ApoTag kit (8 citations) TUNEL apoptosis kit (6 citations)

2 protocols using apotag plus peroxidase in situ tunel apoptosis kit

1

Histological Analysis of Tumor Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumors were fixed in formalin overnight before paraffin embedding. Paraffin blocks were sectioned and stained with H&E at the Dana-Farber/Harvard Cancer Center Rodent Histopathology Core. Immunohistochemistry was carried out using the antibodies Ki67 (Vector), FoxO1, p-Akt (Ser473) (Invitrogen), p-S6rp and p-Erk (Cell Signaling Technology). TUNEL assays were conducted using the ApoTag Plus Peroxidase in situ TUNEL Apoptosis Kit (Millipore) according to the manufacturer’s instructions.
Cheng H., Liu P., Ohlson C., Xu E., Symonds L., Isabella A., Muller W.J., Lin N.U., Krop I.E., Roberts T.M., Winer E.P., Arteaga C.L, & Zhao J.J. (2015). PIK3CAH1047R and Her2 initiated mammary tumors escape PI3K dependency by compensatory activation of MEK-ERK signaling. Oncogene, 35(23), 2961-2970.
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2

Histological Analysis of Tumor Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumors were fixed in formalin overnight before paraffin embedding. Paraffin blocks were sectioned and stained with H&E at the Dana-Farber/Harvard Cancer Center Rodent Histopathology Core. Immunohistochemistry was carried out using the antibodies Ki67 (Vector), FoxO1, p-Akt (Ser473) (Invitrogen), p-S6rp and p-Erk (Cell Signaling Technology). TUNEL assays were conducted using the ApoTag Plus Peroxidase in situ TUNEL Apoptosis Kit (Millipore) according to the manufacturer’s instructions.
Cheng H., Liu P., Ohlson C., Xu E., Symonds L., Isabella A., Muller W.J., Lin N.U., Krop I.E., Roberts T.M., Winer E.P., Arteaga C.L, & Zhao J.J. (2015). PIK3CAH1047R and Her2 initiated mammary tumors escape PI3K dependency by compensatory activation of MEK-ERK signaling. Oncogene, 35(23), 2961-2970.
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