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Cd3ε pacificblue

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CD3ε-PacificBlue is a fluorochrome-conjugated antibody that binds to the CD3 epsilon chain, a component of the T cell receptor complex. This product can be used for the identification and analysis of T cells in flow cytometry applications.

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Lab products found in correlation

Cd38 fitc, by BD (2 mentions) Streptavidin apc, by Thermo Fisher Scientific (2 mentions) Gl7 pe, by BD (2 mentions) Lsrfortessa x 20 instrument, by BD (2 mentions) Ethidium monoazide bromide ema, by Thermo Fisher Scientific (2 mentions) B220 pe cy7, by BD (2 mentions)

2 protocols using cd3ε pacificblue

1

Flow Cytometric Analysis of Lymphocytes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The procedure was nearly identical to the one described22 (link) with a few modifications. Mediastinal lymph nodes or spleens were harvested from euthanized mice at different times after infection or immunization. Single-cell suspensions were stained with the following labeled mAbs: CD3ε-PacificBlue, B220-PECy7, CD38-FITC, GL7-PE (BD Biosciences). rHA was used at 0.1 µg/ml and detected using streptavidin-APC (eBioscience). Cell viability was assessed using 10 µg/ml Ethidium Monoazide Bromide (EMA) (ThermoFisher). Samples were analyzed using a BD LSRFortessa X-20 instrument. Analysis was performed using FlowJo software (TreeStar).
Angeletti D., Gibbs J.S., Angel M., Kosik I., Hickman H.D., Frank G.M., Das S.R., Wheatley A.K., Prabhakaran M., Leggat D.J., McDermott A.B, & Yewdell J.W. (2017). Defining B Cell Immunodominance to Viruses. Nature immunology, 18(4), 456-463.
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2

Flow Cytometric Analysis of Lymphocytes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The procedure was nearly identical to the one described22 (link) with a few modifications. Mediastinal lymph nodes or spleens were harvested from euthanized mice at different times after infection or immunization. Single-cell suspensions were stained with the following labeled mAbs: CD3ε-PacificBlue, B220-PECy7, CD38-FITC, GL7-PE (BD Biosciences). rHA was used at 0.1 µg/ml and detected using streptavidin-APC (eBioscience). Cell viability was assessed using 10 µg/ml Ethidium Monoazide Bromide (EMA) (ThermoFisher). Samples were analyzed using a BD LSRFortessa X-20 instrument. Analysis was performed using FlowJo software (TreeStar).
Angeletti D., Gibbs J.S., Angel M., Kosik I., Hickman H.D., Frank G.M., Das S.R., Wheatley A.K., Prabhakaran M., Leggat D.J., McDermott A.B, & Yewdell J.W. (2017). Defining B Cell Immunodominance to Viruses. Nature immunology, 18(4), 456-463.
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