Paraffin-embedded lung tissue blocks were baked on the hotplate at 75 °C for 20 min and deparaffinized in xylene. The slides were rehydrated from 100%, 90%, to 70% alcohol, and then to PBS. We performed the antigen unmasking using the retriever (Cat. # 62700–10, Electron Microscopy Sciences) with R-Buffer A pH 6.0 (Cat. # 62706–10, Electron Microscopy Sciences) for 2 h to complete the cycle and cool down. Slides were blocked with 20% normal goat serum (NGS) in PBST for 2 h at room temperature. Slides were incubated with an anti-IL-8 antibody (Cat. # 550419, BD Pharmingen™) in 5% NGS with PBS at 4°C for overnight. Slides were washed with PBST and incubated with Alexa 488 coated goat anti-mouse antibody in 5% NGS/PBS for 2 h at room temperature. Slides were washed with PBST and stained with Hoechst (1:5000 in PBS, Invitrogen). Coverslips were mounted on slides using ProLong Glass Antifade Mountant (Cat. # P36982, Invitrogen) and dried out in the dark overnight. Confocal images were acquired using the ZEISS LSM 700 Upright laser scanning confocal microscope and ZEN imaging software (ZEISS).
Anti il 8 antibody
Manufactured by BD
The Anti-IL-8 antibody is a laboratory reagent that binds to and detects the presence of the interleukin-8 (IL-8) protein. IL-8 is a chemokine that plays a role in inflammatory and immune responses. The antibody can be used in various research and analytical applications to measure IL-8 levels in biological samples.
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Lab products found in correlation
2 protocols using anti il 8 antibody
1
Immunofluorescent Staining of IL-8 in Lung Tissue
2
Immunofluorescence Staining of IL-8 in Lung Tissue
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Paraffin-embedded lung tissue blocks were baked on the hotplate at 75°C for 20 min and deparaffinized in xylene. The slides were rehydrated from 100%, 90%, to 70% ethanol and then to PBS. We performed the antigen unmasking using the retriever (Cat. # 62700-10, Electron Microscopy Sciences) with R-Buffer A pH 6.0 (Cat. # 62706-10, Electron Microscopy Sciences) for 2 h to complete the cycle and cool down. Slides were blocked with 20% normal goat serum (NGS) in PBST for 2 h at room temperature. Slides were incubated with an anti-IL-8 antibody (Cat. # 550419, BD Pharmingen™) in 5% NGS with PBS at 4°C overnight. Slides were washed with PBST and incubated with Alexa 488 coated goat anti-mouse antibody in 5% NGS/PBS for 2 h at room temperature. Slides were washed with PBST and stained with Hoechst (1:5000 in PBS, Invitrogen). Coverslips were mounted on slides using ProLong Glass Antifade Mountant (Cat. # P36982, Invitrogen) and dried out in the dark overnight. Confocal images were acquired using the ZEISS LSM 700 Upright laser scanning confocal microscope and ZEN imaging software (ZEISS).
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