Western blotting assays were performed as previously described [16 (link)]. The primary antibodies were as follows: anti-vimentin, anti-FAP, anti-αSMA, anti-LYVE-1, and anti-PAI-1 (Abcam, Cambridge, MA, USA); anti-phospho-AKT (Ser 473), anti-AKT, anti-phospho-ERK1/2 (T202/Y204), anti-ERK1/2, anti-p38, anti-phospho-p38, anti-JNK, anti-phospho-JNK, anti-VE-cadherin, anti-phospho-VE-cadherin (Y685) and anti-GAPDH (CST, Massachusetts, USA). The secondary antibodies were horseradish peroxidase-conjugated anti-rabbit or anti-mouse immunoglobulin-G antibody (Abcam, Cambridge, MA, USA).
Horseradish peroxidase conjugated anti rabbit or anti mouse immunoglobulin g antibody
Manufactured by Abcam
Sourced in United States
Horseradish peroxidase-conjugated anti-rabbit or anti-mouse immunoglobulin-G antibody is a secondary antibody that binds to the primary antibody raised against rabbit or mouse antigens. The horseradish peroxidase enzyme conjugated to the secondary antibody can be used for colorimetric or chemiluminescent detection in various immunoassay techniques.
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2 protocols using horseradish peroxidase conjugated anti rabbit or anti mouse immunoglobulin g antibody
1
Comprehensive Western Blot Analysis
2
Western Blotting of Cell Markers
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Western blotting assay was performed as previously described [18 ]. The primary antibodies were as follows: anti‐CD31, anti‐pan‐CK, antivimentin, anti‐FAP, anti‐αSMA, anti‐LYVE‐1 antibody, antiperiostin (Abcam, Cambridge, MA, USA), anti‐phospho‐AKT (Ser 473), anti‐AKT, anti‐phospho‐ERK1/2 (T202/Y204), anti‐ERK1/2, anti‐Src, anti‐phospho‐Src (Tyr416), anti‐FAK, anti‐phospho‐FAK (Tyr397), anti‐JNK, anti‐phospho‐JNK, anti‐VE‐cadherin, anti‐VE‐cadherin (phospho Y685), and anti‐GAPDH antibody (CST, Danvers, MA, USA). The secondary antibodies were horseradish peroxidase‐conjugated anti‐rabbit or anti‐mouse immunoglobulin‐G antibody (Abcam).
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