Digidoc it system

Manufactured by Analytik Jena

Sourced in Japan, Canada

The DigiDoc-It system is a compact and versatile documentation and imaging device designed for various laboratory applications. It provides high-quality digital imaging capabilities for gel documentation, chemiluminescence detection, and other visualization techniques.

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Lab products found in correlation

Twistamp basic kit, by Twist Bioscience (1 mentions) Epixplore methylated dna enrichment kit, by Takara Bio (1 mentions) Pcr gel dna purification kit, by Nippon Genetics (1 mentions) Applied biosystems sequence scanner software v2, by Thermo Fisher Scientific (1 mentions)

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DigiDoc-it Imaging System (8 citations) DigiDoc-It (5 citations)

2 protocols using digidoc it system

Isothermal RPA with Targeted Enrichment

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RPA was performed using a TwistAmp™ Basic kit (TwistDx, Maidenhead, UK) as follows: a freeze-dried component was reconstituted with 29.5 µl of rehydration buffer and 2.5 µl of each primer (10 µM). A 13.6 µl aliquot was mixed with DNA (20 ng unless stated) to create a pre-reaction mixture. The required blocking agent (0.25–4 µM ORN, 1 µl of CRISPRi RNP complex, 20 or 40 ng of LexA, 0.25–2 µg of MBD2 from the EpiXplore™ Methylated DNA Enrichment Kit (Takara Bio, Shiga, Japan), or 0.5–2 µM 2’3’ddC-modified ODN) and nuclease-free water were added to the pre-reaction mixture to a final volume of 19 µl and incubated at 37 °C for 5 min (pre-incubation). Thereafter, 1 µl of MgOAc (280 mM) was added and the reaction was incubated at 37 °C for a further 30 min. RPA products were purified using a PCR/Gel DNA purification kit (Nippon Genetics, Tokyo, Japan), electrophoresed on 2% or 3% agarose gels, and sequenced if required. DNA gel images were acquired using AE-6905H Image Saver HR (ATTO, Tokyo, Japan) and the DigiDoc-It system (UVP, Cambridge, UK). DNA sequencing data were analyzed using Applied Biosystems Sequence Scanner Software v2.0 (ThermoFisher Scientific, Waltham, MA, USA).

Fujita T., Nagata S, & Fujii H. (2021). Protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations. Communications Biology, 4, 988.

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Polyacrylamide and Agarose Gel Electrophoresis of Aptamers

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Thirty μl rat serum samples with 1 μg/ml of each aptamer in OG or PG were taken from cuvettes following the timed studies and run with 6 μl of 6X loading buffer in a 12% polyacrylamide SDS/HEPES mini Pierce Precise^™ gel (Thermo Fisher) at 125V for 1 h in ice cold SDS/HEPES running buffer. The unstained polyacrylamide gel was removed from its cassette and digitally photographed on a UV transilluminator using a UVP, LLC (Upland, CA), DigiDoc-It^® system. The polyacrylamide gel was then placed in 100 ml deionized water and stained with 5 μl of 1% w/v ethidium bromide (EtBr) for 10 min followed by decanting of the EtBr solution and a 10 min rinse in deionized water. The gel was than photographed again on the UV transilluminator using an orange emission filter. A 1% agarose gel in cold Tris Acetate EDTA (TAE) buffer was also run with 5 μl (5 μg) of the stock 1 mg/ml Eco 3R and ERK aptamers with 5 μl of 6X loading buffer at 100V for 1 h, stained with EtBr and photographed as before. Both gels were run with one lane containing 7 μl of Bio-Rad 50–2,000 base pair (bp) Amplisize^® DNA ladder standards.

Bruno J.G, & Sivils J.C. (2016). Studies of DNA Aptamer OliGreen and PicoGreen Fluorescence Interactions in Buffer and Serum. Journal of fluorescence, 26(4), 1479-1487.

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