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Cd44v6

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CD44v6 is a cell surface glycoprotein that functions as a receptor for hyaluronic acid. It is involved in cell-cell interactions, cell adhesion, and migration.

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Lab products found in correlation

Cd11a, by BD (1 mentions) Hla class 1, by BD (1 mentions) Anti cd10, by BD (1 mentions) Her 2 neu, by BD (1 mentions) Cd206, by BD (1 mentions) Facscanto, by BD (1 mentions) Cd11c, by BD (1 mentions) Hla dr, by BD (1 mentions) Cd133, by BD (1 mentions)

2 protocols using cd44v6

1

Immunophenotyping and FACS of Cancer Cells

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Cells were obtained by trypsinization or using TrypLETM Express from monolayer or sphere culture. The cells were incubated for 30 min at 4°C as follows: CD133-PE-coupled (Cat. No. 12-1338-42 eBioscience), CD44-APC-coupled (559942 BD Pharmingen), and CD44v6 (Cat. No. BMS125 eBioscience). In the case of CD44v6, the cells were washed and incubated with FITC-coupled anti-mouse secondary antibody for 30 min at 4°C in dark conditions. The Single cell suspension was acquired in Attune Nxt cytometer. Data were analyzed using the FlowJo software (Tree Star®). For FACS of cells transduced with the SORE reporter system, RKOCMV and SW480CMV were used as control of fluorescence, and cell sorting was performed using a FACSaria sorter.
Sarabia-Sánchez M.A., Moreno-Londoño A.P., Castañeda-Patlán M.C., Alvarado-Ortiz E., Martínez-Morales J.C, & Robles-Flores M. (2023). Non-canonical Wnt/Ca2+ signaling is essential to promote self-renewal and proliferation in colon cancer stem cells. Frontiers in Oncology, 13, 1121787.
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2

Characterization of Cell Surface Markers

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The following fluorescein (FITC)-, allophycocyanin (APC)-or phycoerythrin (PE)-conjugated mouse anti-human monoclonal antibodies (mAbs) were used: Anti-CD10, -CD11a, CD11c, -CD18, -CD33, -CD40, -CDD44std, -CD44v5, -CD44v6, -CD54, -CD61, -CD62P, -CD86, -CD133, -CD206 (MR), -EGFR, -Her-2/neu, -HLA-DR, HLA class I, -CCR5, -CCR6, Her-2/neu all from BD Pharmingen (San Diego, CA, USA); anti-CD29, -CD36, -CD51, -CD58 from Immunotech (Marseille, France); anti-c-MET, -CCR1, -CCR2, -CCR3, -CCR7, -CXCR1, -CXCR2, -CXCR4 from R&D (Abington, UK); anti-Tag72, -Mucin1 (EMA, CD227), -EMMPRIN from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA) and anti-Epithelial Antigen, -Epithelial Membrane Antigen (EMA) from DAKO (Heverlee, Belgium). Isotype controls included appropriate FITC-, APC- or PE-labeled mouse IgG1, IgG2a or IgG2b. Cells were incubated with mAbs or isotype controls for 20 min at 4°C, washed, resuspended in PBS and analyzed by flow cytometry (FACS Canto; BD Biosciences Immunocytometry Systems, San Jose, CA, USA) using FACS DiVa software.
Mytar B., Stec M., Szatanek R., Węglarczyk K., Szewczyk K., Szczepanik A., Drabik G., Baran J., Siedlar M, & Baj-Krzyworzeka M. (2018). Characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites. Oncology Letters, 15(4), 4849-4858.
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