Binding of B12 to native IL-7Rα expressed at the surface of indicated cells was analyzed by flow cytometry. Cells were incubated with B12 or isotype control at 4°C for 30min, washed with ice-cold PBS and the primary antibody detected using Goat alexa 647-conjugated anti-human (H+L) antibody (ThermoFischer Scientific). Cell viability was determined by forward scatter/side scatter distribution, and by Annexin V (eBioscience) and 7-AAD (BD Bioscience) staining. Samples were acquired using FACS Fortessa I/II (BD Bioscience) and analyzed using FlowJo (Tree Star).
Facs fortessa 1 2
Manufactured by BD
The FACS Fortessa I/II is a flow cytometry instrument manufactured by BD. It is designed to analyze and sort cells based on their physical and fluorescent characteristics. The instrument provides high-performance data acquisition and analysis capabilities for a wide range of applications in fields such as immunology, cell biology, and stem cell research.
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2 protocols using facs fortessa 1 2
1
B12 Binding Assay on IL-7Rα Expressing Cells
2
Quantification of B12 Binding to IL-7Rα
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Binding of B12 to native IL-7Rα expressed at the surface of indicated cells was analyzed by flow cytometry. Cells were incubated with B12 or isotype control at 4 °C for 30 min, washed with ice-cold PBS and the primary antibody detected using Goat alexa 647-conjugated anti-human (H + L) antibody (ThermoFischer Scientific). Cell viability was determined by forward scatter/side scatter distribution, and by Annexin V (eBioscience) and 7AAD (BD Bioscience) staining. Samples were acquired using FACS Fortessa I/II (BD Bioscience) and analyzed using FlowJo (Tree Star).
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