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2 protocols using potassium hydroxide

1

Vaginal Fluid Simulant Preparation

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Lithium chloride (molecular biology grade, L9650) and bovine serum albumin (BSA, ≥ 99%) were purchased from Sigma-Aldrich (St. Louis, MO) and methanesulfonic acid (MSA, 98+%) was purchased from Alfa Aesar (Haverhill, MA). Sodium chloride (USP-grade) and potassium hydroxide (USP-grade) were obtained from BDH through VWR International (Radnor, PA) and calcium hydroxide (98%, extra pure), D,L-lactic acid (85%), and glycerol (Reagent ACS, 99.6%) were obtained from Acros Organics through Thermo Fisher Scientific (Waltham, MA). D-Glucose, monohydrate (biotechnology grade) was obtained from Amresco (Solon, OH) and acetic acid (Certified ACS), urea (reagent grade), and phosphate-buffered saline (PBS, 10× solution, DNase-, RNase-, and protease-free) were purchased from Thermo Fisher Scientific. High purity water (HPW, > 18 MΩ-cm) was obtained from a Milli-Q UF Plus ultrapure water system (EMB Millipore, Billerica, MA). Vaginal fluid simulant (VFS) was prepared according to the recipe by Owen and Katz [36 (link)].
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2

Aflatoxin Standards Characterization

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Aflatoxin B1, B2, G1 and G2 standards (crystalline powder) were purchased from Sigma–Aldrich (St. Louis–MO, USA). Pre–coated TLC plates of silica gel 60 (layer thickness 0.25 mm, 20 x 20 cm) on glass or aluminum, without fluorescent indicator were purchased from E. Merck (Darmstadt, Germany). Analytical grade acetone, acetonitrile, benzene, chloroform, cupric carbonate, ferric chloride, potassium chloride, potassium hydroxide, sodium hydroxide, sodium sulfate, sulfuric acid, xylene and other solvents procured from BDH (Poole, England).
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