The confocal imaging was performed on the Leica TCS SP8 laser scanning confocal microscope with LIAchroic Lightning system and LAS X Lightning Expert super-resolution capacity, 405, 488, 552 and 638 nm excitation diode lasers, with 40×/1.3 oil immersion objective (Leica, Germany). The scanning settings were optimized with Nyquist LAS X function with HyD2.0-SMD excitation sensors, at the regular pinhole size of 0.85 AU, and the scanning frequency at 100 Hz. Each frequency channel was scanned in sequence in order to avoid signal bleeding between the channels. Morphometric analysis was performed automatically and/or manually utilizing LAS X (Leica, Germany) and ImageJ/FIJI. Figures were composed using Adobe Illustrator CC 2017 (Adobe).
Liachroic lightning system
Manufactured by Leica
Sourced in Germany
The LIAchroic Lightning system is a high-performance laboratory equipment designed for various applications. It incorporates a powerful illumination system capable of generating intense and uniform light beams.
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2 protocols using liachroic lightning system
1
High-Resolution Confocal Imaging Protocol
2
Confocal Imaging of Cell Morphology
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The confocal imaging was performed on the Leica TCS SP8 laser scanning confocal microscope with LIAchroic Lightning system and LAS X Lightning Expert super‐resolution capacity, 405, 488, 552, and 638 nm excitation diode lasers, with 40×/1.3 oil immersion objective (Leica, Germany). The scanning settings were optimized with Nyquist LAS X function with HyD2.0‐SMD excitation sensors, at the regular pinhole size of 0.85 AU, and the scanning frequency at 100 Hz. Each frequency channel was scanned in sequence in order to avoid signal bleeding between the channels. Morphometric analysis was performed automatically and/or manually utilizing LAS X (Leica, Germany) and ImageJ/FIJI. Figures were composed using Adobe Illustrator CC 2023 (Adobe).
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