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Cd62l vioblue

Manufactured by Miltenyi Biotec
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CD62L-Vioblue is a fluorochrome-conjugated antibody that specifically binds to the CD62L cell surface antigen. CD62L, also known as L-selectin, is a molecule involved in the homing and migration of lymphocytes. The Vioblue fluorochrome allows for the detection and analysis of CD62L-expressing cells using flow cytometry.

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Lab products found in correlation

Cd3 apc vio770, by Miltenyi Biotec (3 mentions) Cd16 pe, by Miltenyi Biotec (3 mentions) Cd8 viogreen, by Miltenyi Biotec (3 mentions) Cd45ra pe, by Miltenyi Biotec (2 mentions) Cd4 vioblue, by Miltenyi Biotec (2 mentions) Cd45ro apc, by Miltenyi Biotec (2 mentions) Cd56 pe vio770, by Miltenyi Biotec (2 mentions) Ccr7 fitc, by Miltenyi Biotec (2 mentions) Cd19 fitc, by Miltenyi Biotec (2 mentions) Cd34 pe, by Miltenyi Biotec (1 mentions) Celltrace violet, by Thermo Fisher Scientific (1 mentions) Cd4 apc, by Miltenyi Biotec (1 mentions) Cd20 pe vio770, by Miltenyi Biotec (1 mentions) Cd56 pe, by Miltenyi Biotec (1 mentions) Cd3 pe, by Miltenyi Biotec (1 mentions) Cd14 apc, by Miltenyi Biotec (1 mentions) Cd8 apc vio770, by Miltenyi Biotec (1 mentions) Cd45ro pe vio770, by Miltenyi Biotec (1 mentions) Cd45 vioblue, by Miltenyi Biotec (1 mentions) Cd34 apc, by Miltenyi Biotec (1 mentions)

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CD62L (3 citations)

3 protocols using cd62l vioblue

1

Flow Cytometry Immunophenotyping Panel

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MACSQuant Analyzer, MACSQuant Analyzer VYB (Miltenyi Biotec), or FACSCanto, LSRII (Becton Dickinson, NJ) was used to analyze cell populations by flow cytometry. The following antibody and protein conjugates were used: CD3-PE, CD3-APC-Vio770, CD4-APC, CD4-VioGreen, CD8-APC-Vio770, CD8-VioGreen, CD14-APC, CD16-PE, CD19-APC, CD20-PerCP-Vio770, CD20-PE-Vio770, CD34-APC, CD34-PE, CD45-VioBlue, CD45-VioGreen, CD45RO-PE-Vio770, CD56-PE, CD62L-VioBlue, CD95-APC, CD45-VioBlue (mouse) (all from Miltenyi Biotec); ErbB2-Fc fusion protein (R&D Systems), anti-human-IgG (Fc gamma-specific) PE (eBioscience). 7AAD staining was used for dead cell exclusion and Violet Cell Trace (Thermo Fisher) for SupT1 cell tracking.
Radek C., Bernadin O., Drechsel K., Cordes N., Pfeifer R., Sträßer P., Mormin M., Gutierrez-Guerrero A., Cosset F.L., Kaiser A.D., Schaser T., Galy A., Verhoeyen E, & Johnston I.C. (2019). Vectofusin-1 Improves Transduction of Primary Human Cells with Diverse Retroviral and Lentiviral Pseudotypes, Enabling Robust, Automated Closed-System Manufacturing. Human Gene Therapy, 30(12), 1477-1493.
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2

Immunophenotyping of PBMC and T-cells

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Staining of cell surface markers on PBMC and T-cells was performed with CD3-APC/Vio770, CD4-Vioblue, CD8-Viogreen, CD19-FITC, CD56-PE/Vio770, CD16-PE, CD62L-Vioblue, CD45RA-PE, CD45RO-APC, and CCR7-FITC (Miltenyi, San Diego, CA; BD, Franklin Lakes, NJ). All samples were acquired on a MACSQuant Cytometry (Miltenyi) and the data analyzed with Flow Jo (Treestar, Ashland, OR).
McLaughlin L.P., Lang H., Williams E., Wright K.E., Powell A., Cruz C.R., Colberg-Poley A.M., Barese C., Hanley P.J., Bollard C.M, & Keller M.D. (2016). Human Parainfluenza Virus-3 can be Targeted by Rapidly ex vivo Expanded T-Lymphocytes. Cytotherapy, 18(12), 1515-1524.
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3

Comprehensive CBMC and T Cell Immunophenotyping

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Staining of cell surface markers on CBMCs and T cells was performed with CD3-APC/Vio770, CD4-Vioblue, CD8-Viogreen, CD19-FITC, CD56-PE/Vio770, CD16-PE, CD62L-Vioblue, CD45RA-PE, CD45RO-APC, and CCR7-FITC (Miltenyi Biotec and BD Biosciences). 50,000 events per sample were acquired on a MACSQuant cytometer (Miltenyi), and the data were analyzed with Flow Jo (Tree Star).
Dave H., Luo M., Blaney J.W., Patel S., Barese C., Cruz C.R., Shpall E.J., Bollard C.M, & Hanley P.J. (2017). Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood. Molecular Therapy. Methods & Clinical Development, 5, 13-21.
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