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Epcam pe cy7 clone g8

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EPCAM PE/Cy7 clone G8.8 is a fluorescent-labeled antibody that binds to the Epithelial Cell Adhesion Molecule (EPCAM) protein. It is designed for use in flow cytometry applications.

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Lab products found in correlation

Cd45 percp cy5.5 clone 30 f11, by Thermo Fisher Scientific (2 mentions) Cd31 fitc clone 390, by Thermo Fisher Scientific (2 mentions) Gp38 pe clone 8, by Thermo Fisher Scientific (2 mentions) Cyan adp, by Agilent Technologies (2 mentions) Ki67 alexa fluor 647, by BD (1 mentions)

Close spelling variants of this product

EpCAM clone G8.8 EpCAM-PE/Cy7 Epcam (G8.8, Clone G8.8 EpCAM-PE EpCAM

2 protocols using epcam pe cy7 clone g8

1

Multiparametric Flow Cytometry of Cells

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Single cells suspensions were stained for 30 minutes in PBS (with 0.5% BSA and 2mM EDTA) with ‘cocktails’ of the following antibodies CD31-FITC clone 390, gp38-PE clone 8.1.1, CD45 PerCP/CY5.5 clone 30-F11 (from eBiosciences), EPCAM PE/Cy7 clone G8.8 (from Biolegend), Ki67-Alexa 647 and BrDU-Alexa647 (BD Pharmingen). Afterwards cells were washed twice, re-suspended and then analyzed using a Cyan-ADP (Dako) with forward/side scatter gates set to exclude non-viable cells. Data were analyzed with FlowJo software (Tree Star).
Nayar S., Campos J., Chung M.M., Navarro-Núñez L., Chachlani M., Steinthal N., Gardner D.H., Rankin P., Cloake T., Caamaño J.H., McGettrick H.M., Watson S.P., Luther S., Buckley C.D, & Barone F. (2016). Bimodal Expansion of the Lymphatic Vessels Is Regulated by the Sequential Expression of IL-7 and Lymphotoxin α1β2 in Newly Formed Tertiary Lymphoid Structures. The Journal of Immunology Author Choice, 197(5), 1957-1967.
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2

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions were stained for 30 min in PBS (with 0.5% BSA and 2 mM EDTA) with cocktails of the following Abs: CD31-FITC clone 390, gp38-PE clone 8.1.1, CD45 PerCP/Cy5.5 clone 30-F11 (from eBioscience), epithelial cell adhesion molecule (EPCAM) PE/Cy7 clone G8.8 (from BioLegend), Ki67–Alexa Fluor 647, and BrDU–Alexa Fluor 647 (BD Pharmingen). Afterwards cells were washed twice, resuspended, and then analyzed using a CyAn ADP (Dako) with forward/side scatter gates set to exclude nonviable cells. Data were analyzed with FlowJo software (Tree Star).
Nayar S., Campos J., Chung M.M., Navarro-Núñez L., Chachlani M., Steinthal N., Gardner D.H., Rankin P., Cloake T., Caamaño J.H., McGettrick H.M., Watson S.P., Luther S., Buckley C.D, & Barone F. (2016). Bimodal Expansion of the Lymphatic Vessels Is Regulated by the Sequential Expression of IL-7 and Lymphotoxin α1β2 in Newly Formed Tertiary Lymphoid Structures. The Journal of Immunology Author Choice, 197(5), 1957-1967.
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