Casp3 antibody

Cisplatin was purchased from Sigma-Aldrich (St. Louis, MO, USA) and dissolved in normal saline (NS) at a concentration of 5 mmol/L [15 (link)]. Pemetrexed was purchased from Toronto Research Chemicals (Toronto, ON, Canada) and dissolved in dimethyl sulfoxide (DMSO) at a concentration of 10 mmol/L [16 (link)]. Aliquots were stored at -20°C, and stock solutions were diluted to the desired concentrations with growth medium before use. All antibodies used have been previously described [17 (link)]. The antibodies casp8, casp9, PARP, IRE1α and Bip were purchased from Cell Signaling Technology (Danvers, MA, USA). Casp3 antibody was obtained from Imgenex (San Diego, CA, USA). Actin antibody was purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against CHOP and c-FLIP were obtained from Santa Cruz (Santa Cruz, CA, USA) and Alexis (San Diego, CA, USA), respectively. NOXA antibody was purchased from Calbiochem (Merck KGaA, Darmstadt, Germany).

Parthenolide and PMAIP1 antibody were purchased from Calbiochem (Darmstadt, Germany). Briefly, parthenolide was dissolved in dimethyl sulfoxide (DMSO) at a concentration of 10 mmol/L, and the aliquots were stored at -20°C. Stock solutions were diluted to the desired concentrations with growth medium before use. The antibodies of TNFRSF10B and ACTB were purchased from Sigma-Aldrich (St. Louis, MO, USA). CDH1 and CFLAR antibodies were obtained from BD Biosciences (San Jose, CA, USA) and Alexis (San Diego, CA) respectively. Anti-CASP8, CASP9, HSPA5, MCL1, p-EIF2A, and PARP1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). CASP3 anti-body was obtained from Imgenex (San Diego, CA, USA). Antibodies of ATF4, DDIT3 were obtained from Santa Cruz (Santa Cruz, CA).