Resveratrol (C14H12O3; > 98% pure) was purchased from Shaanxi Sciphar Biotechnology Co., Ltd. 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine and polyethylene glycol were purchased from Sigma Inc. Res-NPs were constructed as described in our previous study [12 (link)]. Phosphate-buffered saline (PBS), saline, apoptosis detection kit (Annexin VFITC/PI), protein extraction kit, malondialdehyde (MDA) kit, glutathione peroxidase (GSH-Px) kit, terminal deoxynucleotidyl transferase-mediated deoxyuridine-triphosphate nick end-labeling (TUNEL) apoptotic detection kit, and primary antibodies (BAX, caspase3, and BCL-2) were all purchased from Nanjing Key Gen Biotech. Co., Ltd. Blood urea nitrogen (BUN), C-reactive protein (CRP), cystatin C protease inhibitors (Cys-C), and serum creatinine (SCr) were all obtained from Weiteman, Co., Ltd., (Nanjing, China).
The following instruments were used: automatic biochemical analyzer (Hitachi 7100, Japan), microplate reader (ELx800, BioTek, USA), flow cytometer (FACSCalibur, Becton-Dickinson, USA), inverted fluorescence microscope (IX51, Olympus, Japan), Western electrophoresis apparatus (164-5051, Bio-Rad, USA), spectrophotometer (UV-2540, Shimadzu, Japan), and gel imager (Gel Doc XR, Bio-Rad, USA). Primary antibodies (IGFBP2, AAab136494; KIM-1, ab47634; NGAL, and ab63929) were purchased from Abcam (Cambridge, UK).
The following instruments were used: automatic biochemical analyzer (Hitachi 7100, Japan), microplate reader (ELx800, BioTek, USA), flow cytometer (FACSCalibur, Becton-Dickinson, USA), inverted fluorescence microscope (IX51, Olympus, Japan), Western electrophoresis apparatus (164-5051, Bio-Rad, USA), spectrophotometer (UV-2540, Shimadzu, Japan), and gel imager (Gel Doc XR, Bio-Rad, USA). Primary antibodies (IGFBP2, AAab136494; KIM-1, ab47634; NGAL, and ab63929) were purchased from Abcam (Cambridge, UK).