For the following series of experiments, cells were first cultured in RPMI‐1640 complete media (5% FBS) in 6‐well plates. After reaching confluence, the cells were washed with PBS and cultured in serum‐free media (2 mL/well) for 24 hours. Cell supernatants were collected as the CM and stored at −80°C until use. For collecting CM in chemical inhibition experiments, the PI3K inhibitor LY294002 (Selleckchem, Houston, TX, USA), the HIF‐1α inhibitor PX478 (Selleckchem), the CCR1 antagonist BX471 (Tocris, Bristol, UK), the CCR3 antagonist SB297006 (Tocris), or the CCR5 antagonist DAPTA (Tocris) was used to pretreat cells for 6 hours. The cells were then washed and cultured in serum‐free media for collecting CM.
Sb297006
Manufactured by Bio-Techne
Sourced in United Kingdom
SB297006 is a small molecule inhibitor that targets the protein kinase activity of TGFBR1, also known as ALK5. It is a laboratory research tool used to study the role of the TGF-beta signaling pathway in cellular processes.
Automatically generated - may contain errors
2 protocols using sb297006
1
Conditioned Media Collection and Inhibition
2
Evaluating Small Molecule Inhibitors
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