Hematoxylin counterstain gill s formula

PLLA (221 kDa) was purchased from Corbion (Amsterdam, Netherlands). PEG (35 kDa), hexafluoroisopropanol, and formalin were procured from Sigma Aldrich (St. Louis, MO). Sirolimus, tacrolimus, everolimus, and pimecrolimus (each >99% purity) were purchased from LC Laboratories (Woburn, MA). 10–0 nylon sutures were obtained from either Ethicon (Somerville, NJ) or AROSurgical Instruments (Newport, CA). 1× Dulbecco’s Phosphate-Buffered Saline (PBS) and Tween-20 were purchased from Fisher Scientific (Waltham, MA). Blocking reagent (Background Sniper) was purchased from Biocare Medical (Concord, CA). Anti-CD31 primary antibody (Ab28364) was procured from Abcam (Cambridge, MA) and species appropriate biotinylated secondary antibodies and Horseradish Peroxidase Streptavidin were purchased from Dako (Carpinteria, CA). 3,3-diaminobenzidine and hematoxylin counterstain (Gill’s Formula) were obtained from Vector Laboratories (Burlingame, CA).

PLLA (221 kDa) was purchased from Corbion (Amsterdam, Netherlands). PEG (35 kDa), hexafluoroisopropanol, and formalin were procured from Sigma Aldrich (St. Louis, MO). Sirolimus, tacrolimus, everolimus, and pimecrolimus (each >99% purity) were purchased from LC Laboratories (Woburn, MA). 10–0 nylon sutures were obtained from either Ethicon (Somerville, NJ) or AROSurgical Instruments (Newport, CA). 1× Dulbecco’s Phosphate-Buffered Saline (PBS) and Tween-20 were purchased from Fisher Scientific (Waltham, MA). Blocking reagent (Background Sniper) was purchased from Biocare Medical (Concord, CA). Anti-CD31 primary antibody (Ab28364) was procured from Abcam (Cambridge, MA) and species appropriate biotinylated secondary antibodies and Horseradish Peroxidase Streptavidin were purchased from Dako (Carpinteria, CA). 3,3-diaminobenzidine and hematoxylin counterstain (Gill’s Formula) were obtained from Vector Laboratories (Burlingame, CA).