Male C57/BL6 mice (22-25 g) were obtained from Charles River Laboratories (Wilmington, MA). Rag1−/− mice and wildtype mice were obtained from Jackson laboratories (Bar Harbor, ME). IL-6 enzyme-linked immunosorbent assay (ELISA) kit was obtained from BD Biosciences (San Diego, CA). IL-17 and KC ELISA kits were obtained from R&D Systems (Minneapolis, MN). IL-22 and IL-18 ELISA kits, PE IL-22 (Clone 1H8PWSR, Catalog # 12-7221-82, eBioscience™), eFluor450 IL-17 (Clone eBio17B7 Catalog # 48-7177-82 eBioscience™) and APC-eFluor780 CD3 antibodies (Clone 17A2Catalog # 47-0032-82, eBioscience™), and Fixable Viability Dye eFluor506 were obtained from eBioscience (San Diego, CA). Cell Death Detection ELISA kit was obtained from Roche (Catalog # 11920685001, Indianapolis, IN). FITC-dextran (4D) was obtained from Sigma-Aldrich (St. Louis, MO). Primers to CYP1A1 and β-actin, MirVana miRNA Isolation Kit, High Capacity cDNA Reverse Transcription Kit, and TaqMan Gene Expression Master Mix were obtained from Life Technologies (Grand Island, NY). AHR agonist, 6-formylindolo (3, 2-b) carbazole (FICZ) was obtained from Abeam (Cambridge, MA).
Il 6 enzyme linked immunosorbent assay elisa kit
Manufactured by BD
Sourced in Canada
The IL-6 enzyme-linked immunosorbent assay (ELISA) kit is a laboratory tool used to detect and quantify the levels of the cytokine interleukin-6 (IL-6) in biological samples, such as serum, plasma, or cell culture supernatants. The kit utilizes the ELISA technique to provide a specific and sensitive measurement of IL-6 concentrations.
Automatically generated - may contain errors
Lab products found in correlation
Taqman gene expression master mix, by Thermo Fisher Scientific (1 mentions)
Fixable viability dye efluor 506, by Thermo Fisher Scientific (1 mentions)
Mirvana mirna isolation kit, by Thermo Fisher Scientific (1 mentions)
Wild type mice, by Jackson ImmunoResearch (1 mentions)
High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
Cell death detection elisa kit, by Roche (1 mentions)
Fitc dextran4d, by Merck Group (1 mentions)
Rag1 mice, by Jackson ImmunoResearch (1 mentions)
2 protocols using il 6 enzyme linked immunosorbent assay elisa kit
1
Investigating Inflammatory Responses in Mice
2
Generating IL-6 Expressing Dendritic Cell Vaccines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DCOVA were transfected with AdVIL-6 expressing the transgene IL-6 and control AdVNull without any transgene insert at a multiplicity of infection (MOI) of 150 to form DCOVA/IL-6 and DCOVA/Null vaccines as previously described [52 (link),53 (link)]. The transfected cells were then harvested for phenotypic analysis by flow cytometry. Moreover, the supernatants of DCOVA/IL-6 and DCOVA/Null were assessed for the secretion of IL-6 using the IL-6 enzyme-linked immunosorbent assay (ELISA) kit (BD Bioscience, Mississauga, ON, Canada).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!