Ecb3054d

Normal Human Dermal Fibroblasts (NHDF) were purchased from Sigma (C-12302, Sigma). Cells were cultured in EMEM (ECB2071L, Euroclone), 10% FBS (ECS0180L, Euroclone), 1% NEAA (ECB3054D, Euroclone), 1% 200 mM glutamine (ECB3000D, Euroclone), 1% Penicillin/Streptomycin (ECB3001D, Euroclone). Human aortic smooth muscle cells (HSMC) were purchased from the American Tissue Culture Collection (PCS-100-012, ATCC, Manassas, USA). Cells were cultured in ATCC Vascular Cell Basal Medium (PCS-100-030, ATCC; 500 mL added with 500 μL ascorbic acid, 500 μL rh EGF, 500 μL rh insulin and rh FGF-b, 25 mL glutamine), 5% FBS (ATCC Vascular Smooth Muscle Growth Kit), and 5 mL Penicillin-Streptomycin 100X (Euroclone, Milan, Italy). Human umbilical vein endothelial cells (HUVEC) were purchased from Lonza (Bend, OR, USA) and routinely grown in Endothelial Growth Medium (EGM^™-2) as indicated by the provider. The neuroblastoma cell line SH-SY5Y was purchased from Sigma (ECACC 94030304, Sigma) and cultured in EMEM (ECB2071L, Euroclone) and HAM’S F-12 (ECB7502L, Euroclone) 1:1, 10% FBS (ECS0180L, Euroclone), 1% NEAA (ECB3054D, Euroclone), 1% 200 mM glutamine (ECB3000D, Euroclone), 1% Penicillin/Streptomycin (ECB3001D, Euroclone). All cultures were maintained at 37°C in a 5% CO₂ incubator.