Live-cell imaging. Confocal fluorescence imaging was performed on an IX83/FV3000 confocal laser-scanning microscope (Olympus) equipped with a PlanApo N 60×/1.42 NA oil objective (Olympus), a Z drift compensator system (IX3-ZDC2, Olympus) and stage top incubator (Tokai Hit). Lasers used for excitation were: 405 nm for mTagBFP2; 488 nm for Azami-Green and EGFP; 561 nm for mCherry and Monti-Red; and 640 nm for tdiRFP670 and miRFP670. Live-cell imaging was performed at 37 °C under a humidified preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this this version posted November 10, 2020. ; https://doi.org/10.1101/2020.11.09.375766 doi: bioRxiv preprint 14 5% CO2 atmosphere. Fluorescence and differential interference contrast (DIC) images were analyzed using the Fiji distribution of ImageJ 65 .
The copyright holder for this this version posted November 10, 2020. ; https://doi.org/10.1101/2020.11.09.375766 doi: bioRxiv preprint 14 5% CO2 atmosphere. Fluorescence and differential interference contrast (DIC) images were analyzed using the Fiji distribution of ImageJ 65 .