Kd 804 shodex gel columns

The apparent molar masses and the dispersities of poly(γ-benzyl-L-glutamate)-block-ELP and ELP were determined by size exclusion chromatography (SEC) on a PL GPC50 integrated system from Agilent equipped with two KD-804 Shodex gel columns (300 × 8 mm) (exclusion limits from 4000 Da to 200 000 Da). The detector used in this determination is a refractive index detector (Jasco 1530-RI). Dimethylformamide (DMF) was used as eluent (0.8 mL.min -1 ) at 50 °C in the presence of LiBr (1 g.L -1 ) and the calibration used polystyrene (PS) as standard. Cary 100 UV-Visible spectrophotometer equipped with a multi-cell thermoelectric temperature controller from Agilent Technologies (Les Ulis, FR) and the solutions placed in 1 cm quartz cells. The T t is defined as the temperature corresponding to the maximum of the derivative curve of the absorbance versus temperature, often also named inflection point.

The molar masses and the dispersities of poly(γ-benzyl-L-glutamate)-block-ELP and the ELP precursor were determined by size exclusion chromatography (SEC) on a PL GPC50 integrated system from Agilent equipped with two KD-804 Shodex gel columns (300 x 8 mm) (exclusion limits from 4000 Da to 200 000 Da). The detector used is a refractive index detector (Jasco 1530-RI), dimethylformamide (DMF) has been used as eluent (0.8 mL.min -foot_0 ) at 50 °C in the presence of LiBr (1 g.L -1 ).