Enspire microtiter plate spectrophotometer

For the cell viability assay, the macrophages were seeded in 96-well plates at a concentration of 1 × 10 4 cell per well 1 day before the assay. After the treatment, a cell counting kit 8 (CCK-8; Beyotime Biotechnology) was added to 96-well plates incubated at 37 ℃ for 1-4 h, followed by reading under 450 nm in EnSpire microtiter plate spectrophotometer (PerkinElmer, German). A CellTiter-Lumi Luminescent Cell Viability Assay Kit (Beyotime Biotechnology), which showed the ATP level of cells, was also used to examine the cell viability. After the treatment, 100 µL of the reagent was added to 96-well plates, which contained cells and 100 µL of the medium, and then the luminescence was checked using EnSpire microtiter plate spectrophotometer (PerkinElmer, German).