The indicated cells were treated with CDDP (12.3 μM) for 24 h, then stained by using Annexin V/FITC Cell Apoptosis Kit (KeyGEN BioTECH, Nanjing, China) according to the manufacturer’s instructions. Briefly, indicated cells were washed with PBS and binding solution, subsequently added 5 μl of Annexin V antibody in binding buffer and addition of 2 μl PI, followed by incubation for 15 min. And flow cytometric analysis was performed with a CytoFLEX flow cytometer (BECHMAN COLTER, CA, USA) to determine the percentage of apoptotic cells. Data were analyzed using FlowJo10 (Tree Star, Ashland, Oregon, USA). The experiments were repeated at least three times.
Annexin 5 fitc cell apoptosis kit
Manufactured by Keygen Biotech
Sourced in China
The Annexin V/FITC Cell Apoptosis Kit is a laboratory tool used to detect and quantify apoptosis, a form of programmed cell death. The kit utilizes annexin V, a calcium-dependent phospholipid-binding protein, which has a high affinity for phosphatidylserine (PS) residues. During apoptosis, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by annexin V. The kit also includes FITC (fluorescein isothiocyanate), a fluorescent dye, which is conjugated to annexin V to enable the visualization and quantification of apoptotic cells using flow cytometry or fluorescence microscopy.
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Lab products found in correlation
2 protocols using annexin 5 fitc cell apoptosis kit
1
Apoptosis Analysis of CDDP-treated Cells
2
Cell Cycle and Apoptosis Analysis
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After treating NSCLC cells with chidamide or icotinib alone, or in combination for 48h, cells were washed with phosphate buffer saline (PBS) solution, and incubated in DNA staining solution and permeabilization solution for 30min at room temperature according to the kit instructions (MultiSciences (LIANKE) Biotech Co., Ltd) and then measured by FACS cytometry (BD Biosciences, NJ, USA). The DNA histograms were analyzed using ModFit LT cell cycle analysis software (Verify Software House).
Cell apoptosis was performed using Annexin V-FITC cell apoptosis kit (Keygen Biotech, Nanjing, China) according to the kit instructions. Briefly, after treating with chidamide or icotinib alone, or in combination for 48h, the cells from both suspension and adherence were collected and co-incubated with Annexin V-fluorescein isothiocyanate (FITC) and PI, then measured on a Becton-Dickinson FACScan instrument (BD Biosciences, NJ, USA) using Cell Quest software. This analysis allows discrimination between viable (annexin V-/PI-), early (annexin V+/PI-) and late (annexin V+/PI+) apoptotic cells, as well as necrotic and/or part of late apoptotic (annexin V-/PI+) cells.
Cell apoptosis was performed using Annexin V-FITC cell apoptosis kit (Keygen Biotech, Nanjing, China) according to the kit instructions. Briefly, after treating with chidamide or icotinib alone, or in combination for 48h, the cells from both suspension and adherence were collected and co-incubated with Annexin V-fluorescein isothiocyanate (FITC) and PI, then measured on a Becton-Dickinson FACScan instrument (BD Biosciences, NJ, USA) using Cell Quest software. This analysis allows discrimination between viable (annexin V-/PI-), early (annexin V+/PI-) and late (annexin V+/PI+) apoptotic cells, as well as necrotic and/or part of late apoptotic (annexin V-/PI+) cells.
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