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Anti neuronal class 3 β tubulin

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Anti-Neuronal Class III β-Tubulin is a laboratory reagent used to detect and identify neuronal cells. It is a monoclonal antibody that specifically binds to the class III isoform of the β-tubulin protein, which is predominantly expressed in neurons. This reagent can be used in various techniques, such as immunohistochemistry, immunocytochemistry, and Western blotting, to visualize and analyze neuronal cells.

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Lab products found in correlation

Anti β glactosidase, by Abcam (2 mentions) Dynalight 488, by Abcam (2 mentions) Alexa fluor 546, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Anti e cadherin, by BD (2 mentions) Fitc phalloidin, by Merck Group (1 mentions) Sa00007 1, by Proteintech (1 mentions) Leica dmi8 microscope, by Leica camera (1 mentions) Reddot2, by Biotium (1 mentions) Ab9361, by BD (1 mentions) Anti cytokeratin 5, by Abcam (1 mentions) Alexa fluor 660, by Thermo Fisher Scientific (1 mentions)

3 protocols using anti neuronal class 3 β tubulin

1

Immunofluorescent Labeling of Cochlear Explants

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After treatment, cultured cochlear explants were rinsed and fixed in 4% paraformaldehyde for 15 min. Specimens were subsequently blocked, permeabilized using 5% goat serum, 0.2% Triton X-100, and incubated overnight with anti-Neuronal Class III β-tubulin (1:400, Covance, USA) at 4°C. Next day, the tissues were washed three times and then incubated with Rhodamine conjugated secondary antibody (SA00007-1; Proteintech, China) at a dilution of 1:500 at room temperature for 4 h in darkness. All specimens were labelled with FITC-phalloidin (0.5 g/mL; Sigma-Aldrich), coverslipped, and observed under a fluorescent Leica DMi8 microscope (Leica, Wetzlar, Germany).
Zhao Y., Liang Y., Pan C., Tang X., Sun Y., Xu C., Sun J, & Sun J. (2021). Nicotine induced ototoxicity in rat cochlear organotypic cultures. Translational Neuroscience, 12(1), 407-414.
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2

Whole Mount Immunostaining of Salivary Glands

Cited 1 time
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Whole mount SMG tissues were immunostained as previously described (Sandell et al., 2014 (link)). Primary antibodies used were anti-β-glactosidase (Abcam ab9361) 1:500, anti-E-cadherin (BD Biosciences #610182) 1:50, anti-Neuronal Class III β-Tubulin (Covance, PRB0435-P) 1:1000. Fluorescently conjugated secondary antibodies Alexafluor 488, AlexaFluor 546 (Invitrogen), or Dynalight 488 (Abcam) were used at 1:300. Nuclei were stained with RedDot 2 (Biotium).
Wright D.M., Buenger D.E., Abashev T.M., Lindeman R.P., Ding J, & Sandell L.L. (2015). Retinoic Acid Regulates Embryonic Development of Mammalian Submandibular Salivary Glands. Developmental biology, 407(1), 57-67.
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3

Multicolor Immunofluorescent Staining

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Primary antibodies used were:
anti-β-glactosidase (Abcam ab9361) 1:500,
anti-E-cadherin (BD Biosciences #610182) 1:50,
anti-Neuronal Class III β-Tubulin (Covance, PRB0435-P) 1:1000,
anti-Cytokeratin 5, (Abcam ab24647)1:1000.
anti-Cytokeratin-8 (DSHB TROMA-I)1:50.
Fluorescently conjugated secondary antibodies, each used at 1:300 were: Alexafluor 488, AlexaFluor 546, AlexaFluor 660 (Invitrogen), or Dynalight 488 (Abcam).
Abashev T.M., Metzler M.A., Wright D.M, & Sandell L.L. (2017). Retinoic Acid signaling regulates Krt5 and Krt14 independently of stem cell markers in submandibular salivary gland epithelium. Developmental dynamics : an official publication of the American Association of Anatomists, 246(2), 135-147.
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