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37 protocols using balb c

1

Trichinella pseudospiralis Infection in BALB/c Mice

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Trichinella pseudospiralis (ISS13) was used in the present study. Eight-week-old female BALB/c were obtained from Japan SLC, Inc. All mice were maintained under pathogen-free conditions in individually ventilated cages with standard conditions (23 °C room temperature and 12:12 h light: dark cycle) at the Animal Resources Center of Gifu University Graduate School of Medicine. All animal care and experimental procedures were approved by the Committee for Animal Research and Welfare of Gifu University.
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2

CGRE Deletion Mice for Il-13 Studies

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BALB/c and C57BL/6 mice were purchased from Japan SLC (Hamamatsu, Japan). The conserved GATA3 response element (CGRE) deletion mouse, which lacks the CGRE region on the Il-13 gene, was generated previously [21 (link)]. All animal experiments were performed according to the approved guidelines of the Institutional Review Board of Juntendo University School of Medicine, Tokyo, Japan, and of Tokyo University of Science, Tokyo, Japan. The Animal Care and Use Committees of Tokyo University of Science and of Juntendo University School of Medicine specifically approved this study.
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3

Schistosoma mansoni Infection Model

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A Puerto Rican strain of S. mansoni was maintained in the laboratory by passage between Biomphalaria glabrata snails and ICR mice. BALB/cCrSlc (hereinafter referred to as BALB/c) mice were purchased from Japan SLC (Hamamatsu, Shizuoka, Japan) and maintained in specific pathogen-free conditions at Nagasaki University animal facilities. Provided by Professor Satoshi Uematsu (Osaka City University Graduate School of Medicine, Osaka, Japan), IL-33−/− mice on the BALB/c background were bred and maintained in the same conditions as for WT BALB/c mice at Nagasaki University animal facilities. All mice were provided with water and food ad libitum. Female mice aged 8–12 weeks were subcutaneously infected [34 ] with 50 and 35 freshly shed S. mansoni cercariae for 9 and 12 weeks, respectively. Mice were sacrificed every 3 weeks from week 6 post-infection (6 wpi) onwards, except for worm morphology assessment where mice were also sacrificed at 4 wpi. To assess the production of IL-25, IL-33 and TSLP in intestinal tissues during S. mansoni infection, WT BALB/c mice were infected with S. mansoni cercariae as described above and sacrificed weekly from week 0 to week 4 post-infection, then every 2 weeks to 12 wpi.
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4

Peritoneal Metastasis EOC Mouse Model

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Female nude mice (BALB/c) at 6 weeks of age were obtained from Japan SLC (Nagoya, Japan). The treatment protocol followed the guidelines for animal experimentation adopted by Nagoya University. NOS3TR parental, sh-Ctrl-transfected, and sh-ZEB-transfected cells (7 × 107 cells /0.5 mL of medium/mouse) were injected i.p. to induce the peritoneal metastasis of EOC in the mouse model. The survival time was examined with or without treatment with PTX. In the PTX administration protocol, the intraperitoneal (i.p.) administration of PTX (20 mg/kg body weight), was initiated 48 hrs after tumor inoculation, and repeated once a week a maximum of 3 times. Control mice was administered of PBS only. Survival times were compared among these two groups, respectively.
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5

Murine Models for Autophagy Research

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BALB/c, C57BL/6J (B6), MRL-Fas+/+ (MRL/+), and MRL-Faslpr/lpr (MRL/lpr) mice were purchased from Japan SLC (Hamamatsu, Japan). Green fluorescent protein (GFP)-microtubule-associated protein 1A/1B-light chain 3 (GFP-LC3) mice (25 (link)) were kindly provided by N. Mizushima (The University of Tokyo, Japan).
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6

Mice Models for Ovalbumin Immunology

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Specific-pathogen-free BALB/c (wild type) mice and DO 11.10 TCR Tg mice (ovalbumin (OVA)323-339-specific I-A d -restricted TCR-transgenic mice) were obtained from Japan SLC (Hamamatsu, Japan) and The Jackson Laboratory (Bar Harbor, Maine, USA), respectively, and used at the age of 6 to 8 weeks. They were housed in plastic cages with sterilized paper bedding in a clean, air-conditioned room at 24 C and allowed free access to a standard laboratory diet and water. All procedures performed on the mice were in accordance with the Guidelines of the Animal Care and Use Committee of Meiji Pharmaceutical University, Tokyo.
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7

Schistosoma mansoni Infection in Mice

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A Puerto Rican strain of S. mansoni was maintained in the laboratory by passage between Biomphalaria glabrata snails and ICR mice. BALB/cCrSlc (hereinafter referred to as BALB/c) were purchased from Japan SLC (Hamamatsu, Shizuoka, Japan) and maintained in speci c pathogen-free conditions at Nagasaki University animal facilities. Kindly provided by Professor Satoshi Uematsu (Osaka City University Graduate School of Medicine, Osaka, Japan), IL-33 -/-mice on BALB/c background were bred and maintained in the same conditions as for WT BALB/c at Nagasaki University animal facilities. All mice were provided with water and food ad libitum. Female mice aged 8-12 weeks were subcutaneously infected [34] with 50 and 35 freshly shed S. mansoni cercariae, respectively for nine and 12 weeks. Mice were sacri ced every three weeks from week six, except for worm morphology assessment where mice were also sacri ced at week four of infection. To assess the production of IL-25, IL-33 and TSLP in intestinal tissues during S. mansoni infection, WT BALB/c mice were infected with S. mansoni cercariae as described above and sacri ced weekly from week zero to week four, then fortnightly to week 12 of infection.
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8

Mice Strains for Immunology Research

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C57BL/6, BALB/c, C3H/He, 129X 1/SvJJmsSlc and BALB/cnu/nu mice were purchased from Japan SLC, Inc. C3129F1 mice were obtained by mating female C3H with male 129 mice in our own colony. All animal procedures were approved by the Hokkaido University Animal Care Committee (Approval number: 17–0110) and the methods were performed in accordance with the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health.
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9

BALB/c Mice Acclimatization and Approval

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Female wild-type (BALB/c) and female nude (BALB/c Slc-nu/nu) mice were obtained from Japan SLC (Kyoto, Japan). Animals were allowed to acclimatize for two weeks in the animal facility before any intervention was initiated. The procedures in these experiments were approved by the Nagoya City University Center for Experimental Animal Science, and mice were cared for according to guidelines of the Nagoya City University for Animal Experiments.
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10

BALB/c and C57BL/6 Mice for Tumor Growth

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Seven-week-old female BALB/c and C57BL/6 were purchased from Japan SLC, Inc. The IL12-YFP reporter C57BL/6 mice (B6.129-Il12btm1.1Lky/J) were purchased from the Jackson Laboratory. For tumor growth experiments, each group contained 8 mice except where otherwise specified. All animal experiments were conducted in accordance with institutional guidelines with the approval of the Animal Care and Use Committee of The University of Tokyo and Tokyo University of Science.
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