Fetal bovine serum (FBS) was purchased from AusGeneX (Molendinar, Australia, CAT NO. FBS500-S); DMEM basic (1X) was from Gibco Life Technologies (Shanghai, China); penicillin/streptomycin solution (100 U/mL and 100 mg/mL, respectively), 2′, 7′-dichlorodihydrofluorescein diacetate (DCFH-DA), and Cellular grade DMSO were from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China); 3-(4,5-dimethyl-1,3-thiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide(MTT) was obtained from Biosharp Life Technology Co., Ltd. (Hefei, China); Hoechest 33342, Mito-Tracker Green, and Lyso-Tracker Green were from Beyotime Biotechnology Co., Ltd. (Shanghai, China). All cells were cultured in a CO2 incubator (Wiggens, WCI-180, Beijing, China). All the in vitro fluorescence images experiments were obtained by using an Olympus IX73 + DP73 inverted microscope and laser confocal microscope (Carl Zeiss LSM 900, Jena, Germany). Hypoxic condition was created by coverslips (Citoglas, Hong Kong, China) and a hypoxia chamber (Stemcell Technologies, Cat 27310, Kent, WA, USA). Cytotoxicity assay was performed using an LED light (F&V, Z96kit, Fuijan, China) as irradiation source.
Cellular grade dmso
Manufactured by Solarbio
Sourced in China, United States
Cellular grade DMSO is a highly purified form of dimethyl sulfoxide, a versatile solvent commonly used in cell biology and biomedical research. Its core function is to serve as a cryoprotectant, helping to preserve the viability of cells during freezing and thawing processes.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin solution, by Solarbio (1 mentions)
Lsm 900, by Zeiss (1 mentions)
2 7 dichlorodihydrofluorescein diacetate dcfh da, by Solarbio (1 mentions)
Dmem basic 1x, by Thermo Fisher Scientific (1 mentions)
Hoechest 33342, by Beyotime (1 mentions)
Mito tracker green, by Beyotime (1 mentions)
Lysotracker green, by Beyotime (1 mentions)
Hypoxia chamber, by STEMCELL (1 mentions)
Q exactive orbitrap mass spectrometer, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Super dry solvents, by Energy Chemical (1 mentions)
Agilent 400 dd2 spectrometer, by Agilent Technologies (1 mentions)
2 protocols using cellular grade dmso
1
Cytotoxicity Evaluation of Cells under Hypoxia
2
Solvent Optimization and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All the solvents used in
the reactions are commercially available superdry solvents (Energy
Chemical). The silica gel with a mesh of 200–300 was obtained
from Shanghai Titan Scientific General-Reagent (Shanghai, China).
The eluents were also produced by General-Reagent. The 1H NMR and 13C NMR spectra were recorded at room temperature
using an Agilent 400 DD2 spectrometer (California, USA). The HRMS
was measured using Thermo Scientific Q Exactive Orbitrap Mass Spectrometers
(Massachusetts, USA). All other chemicals were analytical grade.
Fetal bovine serum (FBS) was purchased from AusGeneX (Molendinar,
Australia), RPMI 1640 medium (with penicillin and streptomycin) was
obtained from KeyGEN BioTECH (NanJing, China), trypsin EDTA (0.25%)
was purchased from Gibco Invitrogen (Grand Island, USA), and cellular-grade
DMSO was from Beijing Solarbio Science & Technology Co., Ltd.
(Beijing, China).
the reactions are commercially available superdry solvents (Energy
Chemical). The silica gel with a mesh of 200–300 was obtained
from Shanghai Titan Scientific General-Reagent (Shanghai, China).
The eluents were also produced by General-Reagent. The 1H NMR and 13C NMR spectra were recorded at room temperature
using an Agilent 400 DD2 spectrometer (California, USA). The HRMS
was measured using Thermo Scientific Q Exactive Orbitrap Mass Spectrometers
(Massachusetts, USA). All other chemicals were analytical grade.
Fetal bovine serum (FBS) was purchased from AusGeneX (Molendinar,
Australia), RPMI 1640 medium (with penicillin and streptomycin) was
obtained from KeyGEN BioTECH (NanJing, China), trypsin EDTA (0.25%)
was purchased from Gibco Invitrogen (Grand Island, USA), and cellular-grade
DMSO was from Beijing Solarbio Science & Technology Co., Ltd.
(Beijing, China).
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