Kyse410

Manufactured by American Type Culture Collection

Sourced in United States

KYSE410 is a laboratory equipment product offered by American Type Culture Collection. It is designed for the culturing and maintenance of cell lines. The core function of KYSE410 is to provide a controlled environment for the growth and propagation of various cell types.

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Lab products found in correlation

14 protocols using kyse410

Esophageal Squamous Cell Carcinoma Cell Culture

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Six kinds of ESCC cells (SHEE, KYSE180, TE8, KYSE150, KYSE410 and KYSE520) were purchased from ATCC and cultured in RPMI 1640 medium with 10% fetal bovine serum and 1% antibiotics at 37 °C in 5% CO₂.

Deng Y., Li Y., Wu T., Chen X., Li X., Cai K, & Wu X. (2022). RAD6 Positively Affects Tumorigenesis of Esophageal Squamous Cell Carcinoma by Regulating Histone Ubiquitination of CCNB1. Biological Procedures Online, 24, 4.

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Enrichment and Treatment of ESCC Cells

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Human ESCC cell lines including EC9706, EC109, KYSE410, KYSE150, and KYSE450 were all available from the ATCC (Manassas, VA, USA) for cell culture with 5% CO₂ at 37 °C. RPMI‐1640 medium (Gibco, Carlsbad, CA, USA) was acquired commercially, with 1% Pen/Strep mixture and 10% FBS as supplements. Medium was changed every 3 days. After cells had reached about 80% confluence at the 3rd passage, CD133⁺ cancer cells were obtained by treating with MACS CD133 kit (Miltenyi Biotec, Teterow, Germany). ESCC cells without treatment were termed CD133^‐ cancer cells as control. About 2 mg·mL⁻¹ of actinomycin D was procured from Sigma‐Aldrich (St. Louis, MO, USA) to treat cells.

Wu D., He X., Wang W., Hu X., Wang K, & Wang M. (2020). Long noncoding RNA SNHG12 induces proliferation, migration, epithelial–mesenchymal transition, and stemness of esophageal squamous cell carcinoma cells via post‐transcriptional regulation of BMI1 and CTNNB1. Molecular Oncology, 14(9), 2332-2351.

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Induction of Autophagy and Ferroptosis in Human SCCs

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Human SCCs cell lines (KYSE410 and KYSE450) were purchased from ATCC (Manassas, VA, USA). Cells were maintained in RPMI-1640 (#PM150120; Procell, Wuhan, China) plus 10% fetal bovine serum (FBS; #SH30084.03; Hyclone, South Logan, UT, USA), 100 units/mL penicillin, 100 μg/mL streptomycin. Rapamycin (#ab120224; Abcam, Cambridge, MA, USA), Erastin (#ab209693; Abcam, Cambridge, MA, USA) and Gefitinib (Iressa, AstraZeneca, Macclesfield, UK) were dissolved in dimethyl sulfoxide (DMSO; Sigma, St. Louis, MO, USA) as well as stored at -20°C. To activate autophagy or ferroptosis, cells were administrated with 0.1 μM Rapamycin or Erastin for 16 h.

Chen L., Niu X., Qiao X., Liu S., Ma H., Shi X., He X, & Zhong M. (2022). Characterization of Interplay Between Autophagy and Ferroptosis and Their Synergistical Roles on Manipulating Immunological Tumor Microenvironment in Squamous Cell Carcinomas. Frontiers in Immunology, 12, 739039.

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Profiling Esophageal Squamous Cell Carcinoma

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The tumorous and adjacent normal tissues were collected from 28 ESCC patients in our hospital between March 2020 and March 2021. All participants signed the informed written consent form before this work was conducted. This work was approved by the ethics committee of our hospital (approval number: 2021-KY-0396-007). The clinical characteristics of all the ESCC patients are listed in Table 1.Table 1.

The clinical characteristics of 28 patients with esophageal squamous cell carcinoma

Variable	N (%)
Age (years)
>60	15(53.6)
≤60	13(46.4)
Sex
Male	12(42.9)
Female	16(57.1)
KPS
>80	23(82.1)
≤80	5(17.9)
Pathological T stage
T1	7(25.0)
T2	12(42.9)
T3	9(32.1)
Lymph node metastasis
N1	8(28.6)
N2	10(35.7)
N3	10(35.7)
Histology
Well differentiated	6(21.4)
Moderately differentiated	14(50.0)
Poorly differentiated	8(28.6)
Tumor location
Upper	10(35.7)
Middle	13(46.4)
Lower	5(17.9)

KPS, Karnofsky Performance Status

All cell lines, including ESCC cell lines (KYSE410 and KYSE150), human umbilical vein endothelial cells (HUVECs), and human esophageal epithelial cell line (HET-1A), were obtained from ATCC (USA). The ESCC cell lines were cultured in RPMI-1640 medium, whereas HET-1A and HUVECs cell lines were cultured in the DMEM medium. Cells were cultured using 10% FBS in a CO₂ incubator (5% CO₂) at 37°C.

Shou Y., Wang X., Liang Y., Liu X, & Chen K. (2022). Exosomes-derived miR-154-5p attenuates esophageal squamous cell carcinoma progression and angiogenesis by targeting kinesin family member 14. Bioengineered, 13(2), 4610-4620.

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Analytical Validation of PNA-Based qPCR for KRAS Mutations

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The polypeptide nucleic acid (PNA) based qPCR assay that was used for the detection of KRAS mutations and its analytical validity, sensitivity and specificity have been previously described [20] (link), [21] . The following tumor cell lines, positive for each KRAS point mutation, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and used in control experiments: LS174T (Human colon adenocarcinoma): c.35G > A (p.G12D); HCT116 (Human colon adenocarcinoma): c.38G > A (p.G13D); HUP-T3 (Human pancreatic adenocarcinoma): c.34G > C (p.G12R); KYSE410 (Human esophageal squamous cell carcinoma): c.34G > T (p.G12C); A549 (Human alveolar adenocarcinoma: c.34G > A (p.G12S); SW403 (Human colon adenocarcinoma): c.35G > T (p.G12 V) and RPMI8226 (Human myeloma): c.35G > C (p.G12A)] or wild type for KRAS (HCC827, Human lung adenocarcinoma) at 1:1 and 1:100 concentrations. [20] (link), [21] . In each run, positive and negative controls were included as well as non-template controls. All samples were run in triplicates and a sample was considered as positive if there was at least one positive signal, consistent with previously published studies [22] (link).

Matikas A., Voutsina A., Lagoudaki E., Hatzidaki D., Trypaki M., Stoupis G., Tzardi M., Mavroudis D, & Georgoulias V. (2017). Detection of KRAS Exon 2 Mutations in Circulating Tumor Cells Isolated by the ISET System from Patients with RAS Wild Type Metastatic Colorectal Cancer. Translational Oncology, 10(4), 693-698.

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Cell Line Culture Protocols

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The Human gastric cancer cell lines SNU16, HGC27, MKN45, NCI-N87, KATO III, MKN7, SNU216, MKN74, human esophageal cancer cell lines KYSE-410, OE19 and prostate cell line PC3, were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). All cells except for PC3 were cultured in RPMI 1640 (GIBCO) supplemented with 10% FBS (Invitrogen) and 1% penicillin-streptomycin (Invitrogen). PC3 cells were cultured in DMEM (GIBCO) supplemented with 10% FBS and 1% penicillin-streptomycin. All cells were maintained in a humidified atmosphere of 95% air and 5% CO₂ at 37 °C. Cells were tested negative for mycoplasma contamination.

Wang S., Wu C.Y., He M.M., Yong J.X., Chen Y.X., Qian L.M., Zhang J.L., Zeng Z.L., Xu R.H., Wang F, & Zhao Q. (2024). Machine learning-based extrachromosomal DNA identification in large-scale cohorts reveals its clinical implications in cancer. Nature Communications, 15, 1515.

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ESCC Tumor Tissue Collection Protocol

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Eighty-one pairs of frozen tissues were obtained from patients with ESCC, who underwent radical resections in the Department of Thoracic Surgery of the Cancer Hospital, Chinese Academy of Medical Sciences. The clinical features of the patients are summarized in Supplementary Table 1. All paired tumour and adjacent normal tissues used in this study were collected with informed consent. This study was approved by the Ethics Committee of the National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College. TE1, TE10, KYSE30, KYSE70, KYSE140, KYSE150, KYSE180, KYSE410, KYSE450 and Het-1a cell lines were obtained from American Type Culture Collection.

Wang W., Shao F., Yang X., Wang J., Zhu R., Yang Y., Zhao G., Guo D., Sun Y., Wang J., Xue Q., Gao S., Gao Y., He J, & Lu Z. (2021). METTL3 promotes tumour development by decreasing APC expression mediated by APC mRNA N6-methyladenosine-dependent YTHDF binding. Nature Communications, 12, 3803.

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KRAS-mutant Cancer Cell Lines

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Cancer cell lines harboring KRAS mutations [LS174T, Human colon adenocarcinoma, c.35G>A (p.G12D); HCT116, Human colon adenocarcinoma, c.38G>A (p.G13D); HUP-T3, Human pancreatic adenocarcinoma, c.34G>C (p.G12R); KYSE410, Human oesophageal squamous cell carcinoma, c.34G>T (p.G12C); A549, Human alveolar adenocarcinoma, c.34G>A (p.G12S); SW403, Human colon adenocarcinoma, c.35G>T (p.G12V) and RPMI8226, Human myeloma, c.35G>C (p.G12A)] or wild type for KRAS (HT-29, Human colon adenocarcinoma)] originated from the American Type Culture Collection (ATCC, USA) and were kindly provided from Prof. A. Jung (Institute of Pathology, Ludwig-Maximilian-University, Munich, Germany). All cell lines were cultured in flasks according to supplier's recommendations, before subsequent harvesting using 0.25% trypsin and 5 mmol/L EDTA (GIBCO-BRL). Authentication was done by determining the KRAS mutational status of each cell line by Sanger sequencing. All cell lines except SW403 were heterozygous for KRAS mutations and revealed the expected genotype.

Kalikaki A., Politaki H., Souglakos J., Apostolaki S., Papadimitraki E., Georgoulia N., Tzardi M., Mavroudis D., Georgoulias V, & Voutsina A. (2014). KRAS Genotypic Changes of Circulating Tumor Cells during Treatment of Patients with Metastatic Colorectal Cancer. PLoS ONE, 9(8), e104902.

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Esophageal Cancer Cell Lines Protocol

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Immortalized normal esophageal epithelial cell line (Het-1A) and human ESCC cell lines (TE10, KYSE140, KYSE150, KYSE180, KYSE410, KYSE450, KYSE30, and KYSE70) were purchased from the American Type Culture Collection (ATCC). All cell lines were cultured in RPMI-1640 (Invitrogen) supplemented with 10 % FBS and 1 % penicillin/streptomycin at 37 °C in a humidified 5 % CO₂ incubator. KYSE140 and TE10 were selected for this study from the RT-PCR findings, which showed 10-fold higher expression of Hoxc10 mRNA than in Het1A cells.

He X., Wang H., Wang R., Li Y., Li S., Cao X, & Xu J. (2023). HOXC10 promotes esophageal squamous cell carcinoma progression by targeting FOXA3 and indicates poor survival outcome. Heliyon, 9(10), e21056.

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Esophageal Cell Lines Characterization

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HEEC human normal esophageal epithelial cells and human ESCC cells (KYSE450, KYSE150, KYSE510, KYSE410, KYSE70, and EC9706) were obtained from the American Type Culture Collection (ATCC) and were routinely maintained in high glucose DMEM with 10% fetal bovine serum (FBS). According to the description of the Japanese Collection of Research Bioresources (JCRB) cell bank, KYSE70, KYSE150, and KYSE410 were classified as poorly differentiated cell lines, and EC9706, KYSE450, and KYSE510 were classified as well-differentiated cell lines. Cells were cultured at 37°C in a humidified incubator containing 5% CO₂.

Zhao R., Cao X., Jin S., Li R., Zhong Q., Jiang M., Han J., Guo C, & Zong H. (2020). LncRNA BC200 Promotes Esophageal Squamous Cell Cancer Migration and Invasion and Can Regulate ATF4 Expression. Frontiers in Oncology, 10, 1392.

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