Tecnai 20 electron

Manufactured by Philips

The Tecnai-20 is an electron microscope designed for high-resolution imaging and analysis of materials at the nanoscale. It features a high-brightness electron source, advanced optics, and a stable environmental control system to ensure reliable and consistent performance.

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Tecnai 20 (50 citations) Tecnai-20 electron microscope (6 citations) Tecnai G2 20 STWIN transmission electron microscope (2 citations)

2 protocols using tecnai 20 electron

Ultrastructural Analysis of Neuroblastoma Cells

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Neuroblastoma SH-SY5Y cells of the indicated background were fixed with 1.25% (vol/vol) glutaraldehyde in 0.1 M sodium cacodylate at pH 7.4 for 1 h at room temperature. Thin sections were imaged on a Tecnai-20 electron microscope (Philips-FEI). Morphometric measurements were carried out using ImageJ (National Institutes of Health). For calculations of mitochondria-ER distance, 200 images per condition were considered and a minimum distance of ER located in a 30- or 20-nmn radius from the considered mitochondrion was computed [[42] (link), [43] (link)].

Cerqueira F.M., von Stockum S., Giacomello M., Goliand I., Kakimoto P., Marchesan E., De Stefani D., Kowaltowski A.J., Ziviani E, & Shirihai O.S. (2020). A new target for an old DUB: UCH-L1 regulates mitofusin-2 levels, altering mitochondrial morphology, function and calcium uptake. Redox Biology, 37, 101676.

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Morphological Characterization of Citrate-Stabilized Nanoparticles

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For the morphological characterisation of the citrate stabilized nanoparticles, transmission electron microscopy (TEM) images were acquired at 120 kV acceleration voltage at a FEI Philips Tecnai 20 electron microscope. TEM samples were prepared by dropping ca. 10 µL of the microdroplets emulsion onto a formvar/carbon-coated 150 Cu grid (400 mesh), placed on Parafilm and followed by the evaporation of the solvent at room temperature.

Abalde-Cela S., Taladriz-Blanco P., de Oliveira M.G, & Abell C. (2018). Droplet microfluidics for the highly controlled synthesis of branched gold nanoparticles. Scientific Reports, 8, 2440.

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