Mdf u74v

We recorded descriptive data, including demographic variables, and, for patients, admission diagnosis, disease severity scores (Acute Physiology and Chronic Health Evaluation II score on the day of the study), serum creatinine and 90-day mortality.
Blood samples were drawn at baseline (prior to delivery of the test meal) and at 5, 15, 30, 45, 60, 90, 120, 150, 180, 210 and 240 minutes following the infusion of the test meal. Plasma samples were immediately placed in a cooled storage container and then centrifuged at 3,200 rpm for 15 minutes at 4°C (Universal 320R centrifuge; Hettich, Tuttlingen, Germany). Samples were then decanted and placed in 5-ml collection tubes and stored in a monitored −80°C specimen freezer (MDF-U74V; Sanyo Electric Co, Osaka, Japan) until analysis was performed. Serum samples were kept at room temperature for 30 to 60 minutes to allow clot formation to occur, then centrifuged as above, decanted into 5-ml aliquots and placed into a monitored −80°C specimen freezer. Serum 3-OMG concentrations were measured using liquid chromatography/mass spectroscopy. Citrulline concentrations were determined by stable isotope dilution tandem mass spectrometry (coefficient of variation, <10%). (API 4000; AB SCIEX, Framingham, MA, USA) at the SA Pathology laboratory (Women’s and Children’s Hospital, North Adelaide, SA, Australia) [24 (link)].

Gelatin biomaterial inks containing suture fibers were prepared by adding suture fibers into a gelatin aqueous solution. Briefly, the suture bundles (Vicryl, Ethicon, Somerville, NJ, USA) were cut with surgical blades into filaments each having a length of 3 mm. By using a mortar, the bundles were formed into suture fibers. Gelatin (Bloom 225, type A, MP Biomedicals, Solon, OH, USA) was mixed with distilled water (DIW) at 70 °C for 3 h to form a solution with a final gelatin concentration of 3.5% (w/v) [23 (link)]. The suture fibers and 3.5% gelatin aqueous solution were then mixed at room temperature. Various biomaterial ink formulations containing suture fibers at content of 0% (w/v), 0.1% (w/v), 0.3% (w/v), and 0.5% (w/v), respectively, were prepared. Inclusion of suture fibers in the biomaterial inks was confirmed using scanning electron microscopy (SEM) (CX-200TM; COXEM, Daejeon, Korea). Briefly, the gelatin biomaterial inks containing suture fibers were transferred to a −80 °C deep freezer (MDF-U74V, SANYO, Osaka, Japan) and frozen overnight. The frozen samples were dried by freeze-drying for 3 days. The dried samples were coated with gold using an ion sputter coater (SPT-20; COXEM, Daejeon, Korea) and characterized using SEM at 20 kV.