Fusion tool

PET and CT scans were conducted using Albira PET/SPECT/CT Preclinical Imaging System (Bruker, Billerica, Massachusetts). Animals were anesthetized with isoflurane (induction 4%, maintenance 1.5–2%). 8–10 MBq of [18F]FDG (18F-Fludeoxyglucose) in 100–150 µL was injected intravenously. [18F]FDG was a commercial product Gluscan 500 (Advanced Accelerator Applications Sp. z o. o., Warsaw, Poland). Through the course of radiopharmaceutical uptake and image acquisition respiration was monitored. Scans were started 60 min after [18F]FDG injection. Emission data were collected for 1, 5, 10 min. Spatial resolution of PET images was 1.5 mm. The CT scan parameters ware set as follow: tube voltage was 45 kVp, tube current was 400 µA, and number of projections was 250. Minimal resolution of CT was 90 µm. PET and CT scans were fused using PMOD software, version 3.307, module Fusion Tool (PMOD Technologies LLC, Zurich, Switzerland). Tumor shape on fused image was contoured on all slices consisting part of tumor. Obtained VOIs (volume of interest) were analyzed quantitatively, and SUV (standard uptake value) was calculated.

PET and CT scans were conducted with Albira PET/SPECT/CT Preclinical Imaging System (Bruker, Billerica, MA, USA) with spatial resolution of 1.5 mm for PET and 90 µm for CT. Animals were anesthetized with isoflurane (induction 4%, maintenance 1.5–2%) and 8–13 MBq of [18F]FDG (18F-Fluorodeoxyglucose, Gluscan 500, Advanced Accelerator Applications Sp. z o. o., Warsaw, Poland) was injected intravenously (in a total volume of 100–150 µL). PET/CT scans were started 60 min after [18F]FDG injection and reference CT scans were acquired (tube voltage 45 kVp, tube current 400 µA, number of frames 2, number of projections 600). PET and CT scans were fused using PMOD software, version 3.307, module Fusion Tool (PMOD Technologies LLC, Zurich, Switzerland).