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2 protocols using gelatin

1

TRIM3/BERP Immunohistochemistry in Mouse Brain

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Mice were intracardially perfused with ice-cold PFA (4% in PBS, pH 7.4). Brains were removed, postfixed, embedded in gelatin (Mallinckrodt Baker), and cryostat-sectioned at 40 µm. Sections were stained with anti-TRIM3/BERP (BD Biosciences), and staining was visualized using VECTASTAIN ABC standard kit (Vector Laboratories). Nissl staining was used for gross morphological characterization of brain slices.
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2

Synthesis and Characterization of Europium and Gadolinium Doped Mesoporous Silica Nanoparticles

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98% Tetraethyl orthosilicate (TEOS), (3-Glycidyloxypropyl) trimethoxysilane (GPTMS), ethylenediamine (EDA), 98% Hexadecyl trimethyl ammonium bromide (CTAB), mannose, fucose, glucose, galactose, xylose, McCoy’5A medium and thiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma-Aldrich; 99.9% Europium (III) chloride hexahydrate (EuCl3·6H2O) and 99.9% Gadolinium (III) chloride hexahydrate (GdCl3·6H2O) were purchased from Alfa Aesar; Sodium hydroxide (NaOH), Ethanol (C2H5OH), calcium chloride (CaCl2), barium chloride (BaCl2), gelatin, hydrochloric acid (HCl), sodium sulfate (Na2SO4), trifluoroacetic acid (TFA), 1-Phenyl-3-methyl-5-pyrazolone (PMP), methanol (CH3OH), chloroform (CHCl3), acetonitrile (ACN), sodium nitrate (NaNO3), sodium hydrogen phosphate (Na2HPO4), sodium chloride (NaCl), potassium dihydrogen phosphate (KH2PO4) and dimethyl sulfoxide (DMSO) were purchased from J.T. Baker; potassium chloride (KCl) were purchased from Mallinckrodt Chemical; minimum essential medium (MEM) were purchased from Biowes; fetal bovine serum (FBS), non-essential amino acids (NEAA), antibiotic–antimycotic (AA) and trypsin were purchased Gibco; mouse fibroblasts cell (L929) and human colorectal carcinoma cells (HCT116) were purchased ATCC; Sargassum aquifolium from Kenting-Chuanfan Rock.
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