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Sox10

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Sox10 is a transcription factor that plays a crucial role in the development and maintenance of various cell lineages, including neural crest cells, melanocytes, and Schwann cells. It is a key regulator of gene expression and is involved in processes such as cell differentiation, proliferation, and survival.

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Lab products found in correlation

Glial fibrillary acidic protein (gfap), by Merck Group (2 mentions) Glial fibrillary acidic protein (gfap), by Agilent Technologies (2 mentions) Olig2, by R&D Systems (2 mentions) Axiovert 200m, by Zeiss (2 mentions) Krox 20, by Abcam (1 mentions) Ki67 11f6, by BioLegend (1 mentions) Donkey anti rat rabbit goat, by Jackson ImmunoResearch (1 mentions) Fluoromount g, by Electron Microscopy Sciences (1 mentions) Nis elements software, by Nikon (1 mentions) Confocal microscopy, by Nikon (1 mentions)

3 protocols using sox10

1

Antibody Panel for Cellular Characterization

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The following antibodies were used for ChIP, western blot, and immunofluorescence: GFAP (Chemicon, 1:1000), GFAP (DAKO, 1:1000), HA (Roche, Covance, SCBT), LacZ (Abcam, 1:1000), LacZ (MP, 1:1000), MBP (Covance, 1:500), NFIA (1:2000), Nkx6.1 (DSHB, 1:5), Olig2 (R&D, 1:2000), Pax6 (Abcam, 1:500). PLP (MP, 1:200), S100 (DAKO, 1:1000), Sox10 (1:10000), Sox10 (SCBT 1:100),
Glasgow S., Zhu W., Stolt C.C., Huang T.W., Chen F., LoTurco J.J., Neul J.L., Wegner M., Mohila C, & Deneen B. (2014). Mutual Antagonism Between Sox10 and NFIA Regulates Diversification of Glial Lineages and Glioma Sub-Types. Nature neuroscience, 17(10), 1322-1329.
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2

Antibody Panel for Cellular Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for ChIP, western blot, and immunofluorescence: GFAP (Chemicon, 1:1000), GFAP (DAKO, 1:1000), HA (Roche, Covance, SCBT), LacZ (Abcam, 1:1000), LacZ (MP, 1:1000), MBP (Covance, 1:500), NFIA (1:2000), Nkx6.1 (DSHB, 1:5), Olig2 (R&D, 1:2000), Pax6 (Abcam, 1:500). PLP (MP, 1:200), S100 (DAKO, 1:1000), Sox10 (1:10000), Sox10 (SCBT 1:100),
Glasgow S., Zhu W., Stolt C.C., Huang T.W., Chen F., LoTurco J.J., Neul J.L., Wegner M., Mohila C, & Deneen B. (2014). Mutual Antagonism Between Sox10 and NFIA Regulates Diversification of Glial Lineages and Glioma Sub-Types. Nature neuroscience, 17(10), 1322-1329.
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3

Immunofluorescence Staining of Frozen Sections

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For frozen sections, OCT was removed by incubation with PBS. We permeabilized cells in ice cold MeOH for 10 min., followed by incubation in normal donkey serum (Jackson ImmunoResearch cat# 017–000-121) and 0.3% Triton-X100 (Sigma-Aldrich Cat# X100). Primary antibodies and dilutions were: Ki67 [11F6] (1:200, Biolegend cat# 151202), MBP (1:200 SCBT cat# sc-13,914), Krox20 (1:400 Abcam cat# ab43020), Sox10 (1:100 SCBT cat# sc-17,342), S100 (1:1000 Agilent Technologies Cat# Z031129–2), S100β [EP1576Y] (1:1000 Abcam cat# ab52642) All secondary antibodies were donkey anti Rat/Rabbit/Goat from Jackson ImmunoResearch, reconstituted in 50% glycerol and used at 1:250 dilution. To visualize nuclei, sections were stained with DAPI for 10 min., washed with PBS and mounted in FluoromountG (Electron Microscopy Sciences, Hatfield, PA). Images were acquired with ImageJ Acquisition software using a fluorescence microscope (Axiovert 200 M) with 10x/0.4 or 40x/0.6 objectives (Carl Zeiss, Inc.), or with NIS-Elements software using confocal microscopy (Nikon).
Coover R.A., Healy T.E., Guo L., Chaney K.E., Hennigan R.F., Thomson C.S., Aschbacher-Smith L.E., Jankowski M.P, & Ratner N. (2018). Tonic ATP-mediated growth suppression in peripheral nerve glia requires arrestin-PP2 and is evaded in NF1. Acta Neuropathologica Communications, 6, 127.
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