Fetal bovine serum (FBS), penicillin, streptomycin, lipofectamine 2000, TRIzol reagent, and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-benza-midazolocarbocyanin iodide (JC-1) were obtained from Invitrogen (Carlsbad, CA, USA). RIPA lysis buffer, LDH leakage assay kit, BCA kit, enhanced chemiluminescence (ECL) kit, and antibodies against CD68 and Ly-6G were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Unless otherwise indicated, all chemicals were purchased form Sigma-Aldrich (St. Louis, MO, USA).
Ldh leakage assay kit
Manufactured by Beyotime
Sourced in China
The LDH leakage assay kit is a laboratory equipment used to measure the activity of lactate dehydrogenase (LDH) enzyme released into the culture medium. It provides a quantitative assessment of cell membrane integrity and cytotoxicity.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Enhanced chemiluminescence ecl kit, by Beyotime (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bca kit, by Beyotime (1 mentions)
3 protocols using ldh leakage assay kit
1
Cell Culture Reagent Utilization
2
Mechanically Induced NP Cell Necrosis
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NP cells seeded in 96-well plates were exposed to a mechanical load in a pressure apparatus under the compression of 1 MPa. At the end of treatment, the lactate dehydrogenase (LDH) leakage assay kit (Beyotime, China) was used to detect NP cell necrosis, according to the manufacturer's instructions.
3
Quantifying Cell Damage via LDH Assay
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The extent of cell damage was assessed by measuring the concentration of LDH in the culture medium. Briefly, cells were exposed to non-OGD/R or OGD/R conditions, then the medium was used to assay for LDH leakage with the LDH Leakage assay kit (Beyotime, Shanghai, China) following the manufacturer's instructions. The LDH concentration was determined at 450 nm using a microplate reader (Thermo-fisher, MA, USA).
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