Human Umbilical Artery Endothelial cells (HUAEC) were isolated from umbilical cords of anonymized donors as described71 (link) and according to the principles outlined in the Declaration of Helsinki; this was approved by the ethics board of the WW-University of Münster (2009–537-f-S). HUAEC were cultured in M200 medium with LSGS supplements (Invitrogen) and used for experiments until passage 5. For stimulation with DLL4, cell culture dishes (ibidi) were coated overnight at 4°C with 10 ug/ml DLL4 (R&D) diluted in PBS with 0.2% gelatin (Sigma). The cells were starved overnight in M200 medium supplemented with 0.1% FCS (Sigma) and reseeded on the dishes coated with DLL4 or only gelatin in the starvation medium. . No cell lines used in this study were found in the database of commonly misidentified cell lines that is maintained by ICLAC and NCBI Biosample. The cell lines were not authenticated. The cell lines were not tested for mycoplasma contamination.
Lsgs supplements
Manufactured by Thermo Fisher Scientific
LSGS supplements are a line of laboratory equipment products offered by Thermo Fisher Scientific. These supplements are designed to support the specific needs of laboratory workflows. The core function of LSGS supplements is to provide the necessary components and materials to facilitate various laboratory procedures and experiments.
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Lab products found in correlation
2 protocols using lsgs supplements
1
Isolation and Stimulation of Human Umbilical Artery Endothelial Cells
2
Isolation and Stimulation of Human Umbilical Artery Endothelial Cells
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Human Umbilical Artery Endothelial cells (HUAEC) were isolated from umbilical cords of anonymized donors as described71 (link) and according to the principles outlined in the Declaration of Helsinki; this was approved by the ethics board of the WW-University of Münster (2009–537-f-S). HUAEC were cultured in M200 medium with LSGS supplements (Invitrogen) and used for experiments until passage 5. For stimulation with DLL4, cell culture dishes (ibidi) were coated overnight at 4°C with 10 ug/ml DLL4 (R&D) diluted in PBS with 0.2% gelatin (Sigma). The cells were starved overnight in M200 medium supplemented with 0.1% FCS (Sigma) and reseeded on the dishes coated with DLL4 or only gelatin in the starvation medium. . No cell lines used in this study were found in the database of commonly misidentified cell lines that is maintained by ICLAC and NCBI Biosample. The cell lines were not authenticated. The cell lines were not tested for mycoplasma contamination.
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