Para nitrophenylacetate

Manufactured by Merck Group

Sourced in France

Para-nitrophenylacetate is a laboratory reagent used in various biochemical assays. It is a colorimetric substrate that undergoes enzymatic hydrolysis to produce a yellow-colored product, which can be measured spectrophotometrically. The core function of para-nitrophenylacetate is to serve as a substrate for the detection and quantification of enzymatic activities in research and analytical settings.

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Lab products found in correlation

Propionaldehyde, by Merck Group (4 mentions) Benzaldehyde, by Merck Group (2 mentions) Ab9484, by Abcam (1 mentions) Ab23375, by Abcam (1 mentions) Acetaldehyde, by Merck Group (1 mentions) Peg 3350, by Hampton Research (1 mentions) Para nitrophenyl butyrate, by Merck Group (1 mentions) Pefabloc, by Merck Group (1 mentions) Para nitrophenol, by Merck Group (1 mentions) Para nitrophenyl palmitate, by Merck Group (1 mentions) Azo cm cellulose, by Megazyme (1 mentions) Tetrahydrofuran, by Merck Group (1 mentions)

5 protocols using para nitrophenylacetate

High-throughput ALDH Enzyme Assay

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Benzaldehyde, propionaldehyde, para-nitrophenylacetate, NAD⁺, NADP⁺ and buffers were all purchased from Sigma Aldrich unless otherwise stated. The chemical libraries for performing high throughput screening were purchased from ChemDiv Corp. and ChemBridge Corp. Mafosfamide was bought from Niomech-IIT GmbH, Bielefeld, Germany. Primary ALDH3A1 antibody was purchased from Santa Cruz Biotechnology (sc-67310); primary ALDH1A1 antibody was purchased from Abcam (ab-23375) and primary GAPDH antibody was purchased from Abcam (ab9484).

Parajuli B., Georgiadis T.M., Fishel M.L, & Hurley T.D. (2014). Development of Selective Inhibitors for Human Aldehyde Dehydrogenase 3A1 (ALDH3A1) for the Enhancement of Cyclophosphamide Cytotoxicity. Chembiochem : a European journal of chemical biology, 15(5), 701-712.

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Enzyme Activity Assay Protocol

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All chemicals and reagents including para-nitrophenylacetate, propionaldehyde, NAD⁺, and buffers were purchased from Sigma Aldrich unless where noted otherwise.

Morgan C.A, & Hurley T.D. (2014). Development of a high-throughput in vitro assay to identify selective inhibitors for human ALDH1A1. Chemico-biological interactions, 234, 29-37.

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Analytical Verification of Compounds

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Reagents, including acetaldehyde, propionaldehyde, para-nitrophenylacetate, NAD⁺, and buffers were all purchased from Sigma Aldrich unless otherwise noted. Compounds were purchased from ChemDiv Corp. (San Diego, CA) and were >95% pure based on the NMR spectra provided by the vendor. Following receipt from the vendor, their chemical identities were further verified by LC/MS in the Department of Chemistry, Indiana University-Purdue University Indianapolis and used without further purification.

Morgan C.A, & Hurley T.D. (2015). Characterization of Two Distinct Structural Classes of Selective Aldehyde Dehydrogenase 1A1 Inhibitors. Journal of medicinal chemistry, 58(4), 1964-1975.

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Enzymatic Assay for Aldehyde Dehydrogenase

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Reagents such as benzaldehyde, propionaldehyde, para-nitrophenylacetate, NAD⁺, NADP⁺ and buffers were all purchased from Sigma Aldrich unless otherwise stated. PEG3350 for crystallization trial was purchased from Hampton Research.

Parajuli B., Fishel M.L, & Hurley T.D. (2014). Selective ALDH3A1 Inhibition by Benzimidazole Analogues Increase Mafosfamide Sensitivity in Cancer Cells. Journal of Medicinal Chemistry, 57(2), 449-461.

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Synthesis and Characterization of Polyurethane Derivatives

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Impranil DLN was supplied by Bayer Corporation (Germany). Poly[4-(2,2-dicyanovinyl)-N-bis(hydroxyethyl)aniline-alt-(4,4’-methylenebis(phenylisocyanate)))]urethane and poly[4-(2,2-dicyanovinyl)-N-bis(hydroxyethyl)aniline-alt-(isophroronediisocyanate)]urethane were purchased from Sigma Aldrich (France).
Tween 20, Pefabloc, para-nitrophenyl acetate (pNPA), para-nitrophenyl butyrate (pNPB), para-nitrophenyl palmitate (pNPP), para-nitrophenol (pNP), tetrahydrofuran (THF), 2-sec-butylphenol, (2-Butan-2-ylphenyl), N-methylcarbamate (fenobucarb), Ethyl 2-(4-phenoxyphenoxy)ethylcarbamate (fenoxycarb) and S-Benzyl dipropylthiocarbamate (prosulfocarb) were purchased from Sigma Aldrich (France).
AZCL-xylan (XYL), AZCL-casein (CAS), AZCL-Barley β-glucan (BGLU), AZO-CM Cellulose (CMC), AZO-Carob Galactomannan (GM) were purchased from Megazyme (Ireland).
Lysonase™ Bioprocessing Reagent was purchased from Novagen (France).

Ufarté L., Laville E., Duquesne S., Morgavi D., Robe P., Klopp C., Rizzo A., Pizzut-Serin S, & Potocki-Veronese G. (2017). Discovery of carbamate degrading enzymes by functional metagenomics. PLoS ONE, 12(12), e0189201.

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