Mglu4

Wild-type (mGlu4^+/+), and knockout mice lacking the Grm4 gene (mGlu4^−/−) in C57Bl/6 background were purchased from the Jackson Laboratories. (Strain 8815915) Animals were maintained in a UAB Animal Care Facility. All animal studies were performed following approved guidelines of the Institutional Animal Care and Use Committee. Breeding pairs were used to obtain neonates from both WT and mGlu₄ KO mouse colonies. Homozygous knockout status for the Grm4 gene was confirmed by genomic PCR. ADX88178 was synthesized at Vanderbilt as described in (Le Poul et al. 2012 (link); Yin et al. 2013 (link)). Primary antibodies used in the study were rat anti-mouse MHCII and iNOS from eBioscience. Biotinylated secondary antibody (anti-rat) and Alexa Fluor 488- Streptavidin were purchased from Jackson Immuno Laboratories. An enzyme-linked immunosorbent assay kit for mouse TNFα was purchased from R&D Systems (CA).