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Dulbecco s minimal essential medium dmem

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Dulbecco's minimal essential medium (DMEM) is a cell culture medium that provides essential nutrients and amino acids required for the growth and maintenance of various cell types. It is a widely used medium in cell biology and biotechnology research.

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Lab products found in correlation

Hek293 lentix cells, by Takara Bio (2 mentions) Antibiotic antifungal, by Thermo Fisher Scientific (2 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Minimum essential medium (mem), by Corning (2 mentions) 0.22 μm filter, by Celltreat (2 mentions) Sodium pyruvate, by Corning (1 mentions) Nonessential amino acid, by Corning (1 mentions) Gentamicin, by Thermo Fisher Scientific (1 mentions) Vero 76 cells, by American Type Culture Collection (1 mentions) Egfr inhibitor ag1478, by Merck Group (1 mentions) Sb203580, by Merck Group (1 mentions) Gm6001, by Merck Group (1 mentions) Peroxidase conjugated anti rabbit igg antibody, by Jackson ImmunoResearch (1 mentions) ε aminocaproic acid eaca, by Merck Group (1 mentions) Total erk 1 2 antibody, by BD (1 mentions) Phosphatidylinositol specific phospholipase c, by Merck Group (1 mentions) Dulbecco s phosphate buffered saline dpbs, by Corning (1 mentions) Pd98059, by Merck Group (1 mentions) Sp600125, by Merck Group (1 mentions)

Spelling variants of this product

DMEM medium (233 citations) Minimal Essential Medium (11 citations) Dulbecco’s Minimal Essential Medium (7 citations)

5 protocols using dulbecco s minimal essential medium dmem

1

Propagation and Characterization of EEEV

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Eastern equine encephalitis virus isolate V105-00210 was obtained from internal USAMRIID collection. The virus (Vero-1) was received from the Centers for Disease Control and Prevention (CDC) Fort Collins, CO [17 (link)]. The virus stock was passed in Vero-76 cells (American Type Culture Collection, ATCC; Bethesda, MD) twice for the production of Master (Vero-2) and Working (Vero-3) virus stocks. The virus stock was deep sequenced to verify genomic sequence and to ensure purity. In addition, the stock was tested and determined to be negative for both endotoxin and mycoplasma.
Vero-76 cells were propagated at 37°C with 5% CO2 in Dulbecco’s Minimal Essential Medium (DMEM) (CellGro) containing 2% (v/v) fetal bovine serum (FBS) (Hyclone), sodium pyruvate (1 mM) (CellGro), 1% (v/v) non-essential amino acids (CellGro), and 50 μg/mL gentamicin (Invitrogen).
Trefry J.C., Rossi F.D., Accardi M.V., Dorsey B.L., Sprague T.R., Wollen-Roberts S.E., Shamblin J.D., Kimmel A.E., Glass P.J., Miller L.J., Burke C.W., Cardile A.P., Smith D.R., Bavari S., Authier S., Pratt W.D., Pitt M.L, & Nasar F. (2021). The utilization of advance telemetry to investigate critical physiological parameters including electroencephalography in cynomolgus macaques following aerosol challenge with eastern equine encephalitis virus. PLoS Neglected Tropical Diseases, 15(6), e0009424.
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2

Inhibition of MAPK Signaling Pathways

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suPAR and ATF were purchased from American Diagnostica, Inc. (Greenwich, CT). fMLP, Pertussis toxin (Gαi inhibitor, 100ng/ml), Phosphatidylinositol-specific-phospholipase C (100µg/mL), PD98059 (ERK inhibitor, 25µM) SB203580 (p38MAPK inhibitor, 10µM), SP600125 (JNK inhibitor, 1µM), EGFR inhibitor ( AG1478, 10µM), ε-aminocaproic acid (EACA; plasmin inhibitor, 100µM), aprotinin (plasmin inhibitor, 100 units/ml) and GM6001 (MMP inhibitor, 10nM) were purchased from Sigma Chemical Co. (St. Louis, MO). Peroxidase-conjugated anti-rabbit IgG antibody (raised in goat) and peroxidase-conjugated anti-mouse IgG antibody (raised in goat) were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA). Phospho-ERK1/2 antibody was purchased from Promega, Inc. (Madison, WI). Total ERK 1/2 antibody was purchased from BD Transduction Laboratories (Lexington, KY). Phospho-p38MAPK and phospho-JNK antibodies were purchased from Biosource (Camarillo, CA). Total p38MAPK and JNK antibodies were purchased from Cell Signaling (Beverley, MA). Dulbecco’s minimal essential medium (DMEM) and Dulbecco’s phosphate buffered saline (dPBS) were purchased from Corning Cellgro (Corning, NY).
Duru E.A., Fu Y, & Davies M.G. (2015). ROLE OF FORMIC RECEPTORS IN SOLUBLE UROKINASE RECEPTOR INDUCED HUMAN VASCULAR SMOOTH MUSCLE MIGRATION. The Journal of surgical research, 195(2), 396-405.
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3

Bladder Cancer Cell Line Characterization

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All cells were cultured in Dulbecco’s Minimal Essential Medium (DMEM; Corning, Manassas VA) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin/Fungizone (Corning, Manassas, VA). Cell lines 253J, 5637, J82, RT4, SW780 and T24 were obtained from the American Type Culture Collection (ATCC, Manassas, VA). UM-UC-1,3,5,6,7,9,10,12,13,14,15,17 and 18 were obtained from the originator, Dr. H. Barton Grossman, The University of Texas-MD Anderson Cancer Center, Houston, TX. All cell lines were appropriately characterized or found to be unique by DNA short tandem repeat analysis (IDEXX Bioresearch, Columbia, MO) within 6 months of use. All cultures were monitored and found to be free of bacterial and mycoplasma infection (last tested 2017/8/24).
Tamura S., Wang Y., Veeneman B., Hovelson D., Bankhead A I.I.I., Broses L.J., Lorenzatti Hiles G., Liebert M., Rubin J.R., Day K.C., Hussain M., Neamati N., Tomlins S., Palmbos P.L., Grivas P, & Day M.L. (2018). Molecular Correlates of In Vitro Responses to Dacomitinib and Afatinib in Bladder Cancer. Bladder Cancer (Amsterdam, Netherlands), 4(1), 77-90.
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4

Propagation of SVG-A and HEK293-LentiX Cells

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SVG-A is a transformed cell line derived from primary fetal human glial cells and was described previously (Major et al., 1985 (link)). SVG-A cells were grown in minimal essential medium (MEM) (Corning, Inc, New York, NY) supplemented with 10% fetal bovine serum (FBS) (Atlanta Biologicals, Flowery Branch, GA) and 1% antifungal/antibiotic (Gibco Life Technologies, Gaithersburg, MD). HEK293-LentiX cells are a subclone of the HEK293 line optimal for lentiviral packaging systems and were obtained from (Takara). LentiX cells were grown in Dulbecco’s minimal essential medium (DMEM) (Corning) supplemented with 10% FBS, 1% nonessential amino acids (Gibco Life Technologies), and 1% antifungal/antibiotic. EV-depleted media was used as needed for EV-related experiments while complete media was used for general passage of cell lines. EV-depleted media was produced as described (Théry et al., 2002 ). Briefly, 2X media was prepared and spun at 100,000 ×g in a Type 45 Ti rotor (k-factor = 133), for 18 hours at 4°C. Media was then diluted before use to 1X and filtered through 0.22 μm filter (CELLTREAT, Pepperell, MA). Cells were grown in a humidified chamber at 37°C and 5% CO2.
Generation of the JC polyomavirus strain Mad-1/SVEΔ and production and purification of viral stocks was previously described (Liu & Atwood, 2000 , Liu et al. ).
Morris-Love J., O’Hara B.A., Gee G.V., Dugan A.S., O’Rourke R.S., Armstead B.E., Assetta B., Haley S.A, & Atwood W.J. (2022). Biogenesis of JC polyomavirus associated extracellular vesicles. Journal of extracellular biology, 1(5), e43.
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5

Propagation of SVG-A and HEK293-LentiX Cells

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SVG-A is a transformed cell line derived from primary fetal human glial cells and was described previously (Major et al., 1985 (link)). SVG-A cells were grown in minimal essential medium (MEM) (Corning, Inc, New York, NY) supplemented with 10% fetal bovine serum (FBS) (Atlanta Biologicals, Flowery Branch, GA) and 1% antifungal/antibiotic (Gibco Life Technologies, Gaithersburg, MD). HEK293-LentiX cells are a subclone of the HEK293 line optimal for lentiviral packaging systems and were obtained from (Takara). LentiX cells were grown in Dulbecco’s minimal essential medium (DMEM) (Corning) supplemented with 10% FBS, 1% nonessential amino acids (Gibco Life Technologies), and 1% antifungal/antibiotic. EV-depleted media was used as needed for EV-related experiments while complete media was used for general passage of cell lines. EV-depleted media was produced as described (Théry et al., 2002 ). Briefly, 2X media was prepared and spun at 100,000 ×g in a Type 45 Ti rotor (k-factor = 133), for 18 hours at 4°C. Media was then diluted before use to 1X and filtered through 0.22 μm filter (CELLTREAT, Pepperell, MA). Cells were grown in a humidified chamber at 37°C and 5% CO2.
Generation of the JC polyomavirus strain Mad-1/SVEΔ and production and purification of viral stocks was previously described (Liu & Atwood, 2000 , Liu et al. ).
Morris-Love J., O’Hara B.A., Gee G.V., Dugan A.S., O’Rourke R.S., Armstead B.E., Assetta B., Haley S.A, & Atwood W.J. (2022). Biogenesis of JC polyomavirus associated extracellular vesicles. Journal of extracellular biology, 1(5), e43.
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