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Thromboplastin

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Thromboplastin is a laboratory reagent used in coagulation testing. It is a crucial component in the prothrombin time (PT) test, which is used to evaluate the extrinsic pathway of the blood clotting process. Thromboplastin contains tissue factor, calcium, and phospholipids, which together initiate the clotting cascade and allow for the measurement of the time it takes for a clot to form.

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Lab products found in correlation

Sephacryl s 300, by Merck Group (1 mentions) Fibrinogen, by Merck Group (1 mentions)

5 protocols using thromboplastin

1

Thromboplastin-Induced Micro Emboli Model

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We utilized a model similar to that described previously30 (link). Thromboplastin (Sigma, St Louis, MO) used for these studies was from rabbit brain. Each vial of Thromboplastin (3–4 mg) was resuspended in 4 mL saline and 100 µL of this solution was injected per mouse as below. The dose range examined had a 100% survival rate. As described previously female mice were used as preliminary studies revealed a sex difference in sensitivity to Thromboplastin39 (link). Briefly, anesthesia was induced in Foxn1nu or C57BL/6J subjects with 3–4% isoflurane having appropriate oxygen flow. Once animals reached deep anesthesia, they were maintained at 2% isoflurane with continuous monitoring. With the animals under deep anesthesia, Thromboplastin (4 mg/ml) isolated from rabbit brain (Sigma Aldrich) was administered via tail vein injection to reach sub-lethal doses in each mouse while triggering the formation of micro emboli. After 10 minutes from the initial injection, PAR1-RIP (10µM/kg) prepared in PBS Buffer (pH 7.4) containing PEG 16000 and no more than 15% DMSO was injected via tail vein to reach a final concentration of 500 picomoles in each mouse.
Page M.J., Lourenço A.L., David T., LeBeau A.M., Cattaruzza F., Castro H.C., VanBrocklin H.F., Coughlin S.R, & Craik C.S. (2015). Non-invasive imaging and cellular tracking of pulmonary emboli by near-infrared fluorescence and positron-emission tomography. Nature Communications, 6, 8448.
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2

Thromboplastin-Induced Microemboli Formation

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We used a model similar to that described previously30 (link). Thromboplastin (Sigma-Aldrich) used for these studies was from rabbit brain. Each vial of Thromboplastin (3–4 mg) was resuspended in 4 ml saline and 100 μl of this solution was injected per mouse as below. The dose range examined had a 100% survival rate. As described previously female mice were used as preliminary studies revealed a sex difference in sensitivity to Thromboplastin40 (link). Briefly, anaesthesia was induced in Foxn1nu or C57BL/6J subjects with 3–4% isoflurane having appropriate oxygen flow. Once animals reached deep anaesthesia, they were maintained at 2% isoflurane with continuous monitoring. With the animals under deep anaesthesia, Thromboplastin (4 mg ml−1) isolated from rabbit brain (Sigma-Aldrich) was administered via tail-vein injection to reach sub-lethal doses in each mouse while triggering the formation of micro emboli. After 10 min from the initial injection, PAR1–RIP (10 μM kg−1) prepared in PBS (pH 7.4) containing polyethylene glycol 16,000 and no more than 15% dimethylsulphoxide (DMSO) was injected via tail vein to reach a final concentration of 500 picomoles in each mouse.
Page M.J., Lourenço A.L., David T., LeBeau A.M., Cattaruzza F., Castro H.C., VanBrocklin H.F., Coughlin S.R, & Craik C.S. (2015). Non-invasive imaging and cellular tracking of pulmonary emboli by near-infrared fluorescence and positron-emission tomography. Nature Communications, 6, 8448.
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3

Thromboplastin and Collagen/Epinephrine Induced Pulmonary Embolism

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Thromboplastin induced pulmonary embolism (PE) was achieved through the I.V injection of 5 μL Thromboplastin [Sigma] and mice were monitored for 30-minutes for breathing cessation after which mortality was recorded and lungs were collected for histology. Collagen/epinephrine PE was induced as in ref(Rowley et al., 2016 (link)) using 64 mg/kg of collagen and 5.4 mg/kg of epinephrine and anti-TLT-1 (100ug/100ul) 20 minutes prior to the collagen/epinephrine injection.
Jessica M.O., Fiorella R., Ocatavio S., Linnette R., Nahomy L., Kanth M.B., Bismarck M., Rondina. M.T, & Valance W.A. (2018). TLT-1-CONTROLS EARLY THROMBUS FORMATION AND STABILITY BY FACILITATING AIIBB3 OUTSIDE-IN SIGNALING IN MICE.. International journal of advanced research, 6(3), 1143-1149.
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4

Purification and Characterization of Proteins

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Phosphatydylcholine, Triton X-100, phenol red, Dithiothreitol (DTT), Phenyl methyl sulfonyl fluoride (PMSF), 1,10 Phenanthroline, bovine serum albumin (BSA), Diethylaminoethyl cellulose (DEAE-Cellulose), marker proteins, Sephacryl S-300, and thromboplastin were from Sigma Chemical (St. Louis, USA). The other chemicals were of analytical grade. Human blood samples were obtained from the laboratory of medical center hospital of our institute.
Darwish D.A., Masoud H.M., Abdel-Monsef M.M., Helmy M.S., Zidan H.A, & Ibrahim M.A. (2021). Phospholipase A2 enzyme from the venom of Egyptian honey bee Apis mellifera lamarckii with anti-platelet aggregation and anti-coagulation activities. Journal of Genetic Engineering & Biotechnology, 19, 10.
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5

Recombinant HV-Based Biomaterial

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Recombinant HV was provided by B. He from Nanjing Tech University. AAm and N,N′-methylene-bisacrylamide (MBA) were purchased from Aladdin. The TCP cross-linker and the clot-targeted GGCR(NMe)EKA peptide were purchased from GL Biochem. Fibrinogen and thromboplastin were purchased from Sigma-Aldrich.
Xu X., Huang X., Zhang Y., Shen S., Feng Z., Dong H., Zhang C, & Mo R. (2020). Self-regulated hirudin delivery for anticoagulant therapy. Science Advances, 6(41), eabc0382.
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