R26r confetti

Manufactured by Jackson ImmunoResearch

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R26R-Confetti is a reporter mouse line that expresses a Cre-dependent multicolor fluorescent protein expression cassette. It is used to lineage trace and visualize the fate of Cre-expressing cells and their progeny.

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7 protocols using r26r confetti

Genetic Lineage Tracing in Mice

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All aspects of mouse experiments were approved by the Finnish National Board of Animal Experimentation (ESAVI/1284/04.10.07/2016). The plug day was considered as embryonic day E0 and the date of birth as post-natal day P0. All embryos were staged according to limb morphological criteria.
Wild-type ICR mice and Rosa-R26R reporter transgenic mice [R26R-TdTomato (Madisen et al., 2010 (link)), R26R-Confetti (Snippert et al., 2010 (link)), and R26R-mT/mG (Muzumdar et al., 2007 (link))] were purchased from Jackson Laboratory, USA. Fucci (Sakaue-Sawano et al., 2008 (link)) fluorescent cell cycle reporter mouse strain was used for cell cycle analyses. The reporter lines were crossed with the K14-Cre43 line (Andl et al., 2004 (link)), kindly provided by Dr. Sarah Millar, to induce genetic recombination. R26R-mT/mG mice were crossed with aSMA-Cre line (LeBleu et al., 2013 (link)), kindly provided by Prof. Kari Alitalo, for recombination tests.
All tissues were fixed with 4% paraformaldehyde (PFA). For E17 and E18 stages, tissues were decalcified with EDTA prior embedding. For histology and immunohistochemistry, tissues were embedded in paraffin and 5 μm thickness sections were used.

Kuony A, & Michon F. (2017). Epithelial Markers aSMA, Krt14, and Krt19 Unveil Elements of Murine Lacrimal Gland Morphogenesis and Maturation. Frontiers in Physiology, 8, 739.

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Genetic Tracing of Cardiac Development

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Mouse strains Numb^fl/fl&Numblike^fl/fl (65 (link), 76 (link)), Cdh2^fl/fl (49 (link)), Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)} (mTmG) (53 (link)), Rosa26^CreERT2 (63 (link)), and R26R-Confetti (64 (link)) were purchased from The Jackson Laboratory. Robert Schwartz, University of Houston, Houston, TX, provided Nkx2.5^Cre/+ mice (41 (link)). The aMHC-cN-cadherin transgene was injected into fertilized oocytes as previously described (67 (link)). Embryos harvested at around noon on embryonic day 9 were counted as E9.5, and those harvested at around 6 PM were counted as E9.75. All animal experiments are approved by the Institutional Animal Care and Use Committee at Albany Medical College and performed according to the Guide for the Care and Use of Laboratory Animals (77 ).
Various experimental protocols were applied in this study, and most of the experiments were briefly introduced in the text to make the content easier to be understood. The details of each protocol are available in SI Appendix.

Miao L., Li J., Li J., Lu Y., Shieh D., Mazurkiewicz J.E., Barroso M., Schwarz J.J., Xin H.B., Singer H.A., Vincent P.A., Zhong W., Radice G.L., Wan L.Q., Fan Z.C., Huang G, & Wu M. (2019). Cardiomyocyte orientation modulated by the Numb family proteins–N-cadherin axis is essential for ventricular wall morphogenesis. Proceedings of the National Academy of Sciences of the United States of America, 116(31), 15560-15569.

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Mouse Strain Acquisition and Use

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The following mouse strains were purchased from the Jackson Laboratory (Bar Harber, ME): Bmi1Cre^ER26 (link), R26R-YFP27 (link), R26R-Confetti34 (link), Ptenf/f35 (link) and Bmi1-GFP25 (link). Nkx3.1Cre^ERT2 mice5 (link) were the kind gift of M. Shen (Columbia University). All mouse studies were approved by the Northwestern University Institutional Animal Care and Use Committee.

Yoo Y.A., Roh M., Naseem A.F., Lysy B., Desouki M.M., Unno K, & Abdulkadir S.A. (2016). Bmi1 marks distinct castration-resistant luminal progenitor cells competent for prostate regeneration and tumour initiation. Nature Communications, 7, 12943.

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Induction of Muscle Tumors in Mice

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The R26R-Confetti (stock no. 013731) and R26R-LSL-DTR (stock no. 007900) mice were obtained from The Jackson Laboratories. The Kras^LSL-G12D mice were provided by Tyler Jacks (MIT, Cambridge, Massachusetts, USA), and the Trp53^f/f mice were provided by Anton Berns (NKI, Amsterdam, The Netherlands). All mice were on a mixed genetic background, and both male and female mice were included in the study. The animals were housed at room temperature with a 12-hour-light/dark cycle. To induce tumors, adenovirus-expressing Cre recombinase mixed with 50 μl of 2 M CaCl₂ in DMEM were directly injected into the hind gastrocnemius muscle of 7- to 12-week-old mice. Growth of tumors was confirmed by physical examination at the injection site and by histology.

Tang Y.J., Puviindran V., Xiang Y., Yahara Y., Zhang H., Nadesan P., Diao Y., Kirsch D.G, & Alman B.A. (2021). Tumor-propagating side population cells are a dynamic subpopulation in undifferentiated pleomorphic sarcoma. JCI Insight, 6(22), e148768.

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Conditional Ezh2(Y641F) Knock-In Mouse Model

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Animal care was in strict compliance with institutional guidelines established by the Weill Cornell Medical College, the Guide for the Care and Use of Laboratory Animals (National Academy of Sciences 1996), and the Association for Assessment and Accreditation of Laboratory Animal Care International. Conditional Ezh2(Y641F)^fl knock-in model was generated in our lab (Beguelin et al., 2016 (link)). By crossing Ezh2(Y641F)^fl with the transgenic Cγ1-cre strain (The Jackson Laboratory, stock 010611) we generated heterozygous mice. As control group, we used EZH2(Y641F)^WT Cγ1-cre positive littermates. The following strains were obtained from Jackson Laboratory: C57Bl/6J (CD45.2, stock 000664), B6.SJL-Ptprc^aPepc^b/Boy (CD45.1, stock 002014), Rag1 KO (stock 002216), R26-lox-stop-lox-YFP (stock 006148), OT-II (stock 004194), R26R-Confetti (stock 013731), GFP-Myc (stock 021935). R26-Fucci2aR was developed by J. Jackson group (Mort et al., 2014 (link)).

Béguelin W., Teater M., Meydan C., Hoehn K.B., Phillip J.M., Soshnev A.A., Venturutti L., Rivas M.A., Calvo-Fernández M.T., Gutierrez J., Camarillo J.M., Takata K., Tarte K., Kelleher N.L., Steidl C., Mason C.E., Elemento O., Allis C.D., Kleinstein S.H, & Melnick A.M. (2020). MUTANT EZH2 INDUCES A PRE-MALIGNANT LYMPHOMA NICHE BY REPROGRAMMING THE IMMUNE RESPONSE. Cancer cell, 37(5), 655-673.e11.

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Spatiotemporal Transcriptomic Mapping of Mouse Tissues

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All mouse work was performed in accordance with the Institutional Animal Care and Use Committees (IACUC) and relevant guidelines of Boston Children’s Hospital and Masaryk University.
Embryonic day (E) 16.5 embryos were obtained from time-pregnant CD-1 dams for embryonic scRNA-seq analyses. For snRNA-seq analyses, E16.5 embryos, adult (4 months) and aged (20 months) C57BL/6J males and females were used. All animals were housed under 12hr/12hr day night cycle with access to standard chow and water ad libitum.
CD1(ICR)– Charles River Laboratories Strain 022.
Cx3cr1-GFP (catalog #: 005582, The Jackson Laboratory)
C57BL/6 – Charles River Laboratories
C57BL/6J – (catalog #: 000664, The Jackson Laboratory)
Wnt1-Cre2 – (catalog #: 002137, The Jackson Laboratory)
R26R-Confetti – (catalog #: 013731, The Jackson Laboratory)

Dani N., Herbst R.H., McCabe C., Green G., Kaiser K., Head J., Cui J., Shipley F.B., Jang A., Dionne D., Nguyen L., Rodman C., Riesenfeld S.J., Prochazka J., Prochazkova M., Sedlacek R., Zhang F., Bryja V., Rozenblatt-Rosen O., Habib N., Regev A, & Lehtinen M.K. (2021). A cellular and spatial map of the choroid plexus across brain ventricles and ages. Cell, 184(11), 3056-3074.e21.

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Genetically Modified Mouse Strains for Platelet and Hemostasis Research

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Mouse strains PF4-Cre, R26R-Confetti, PC-G5-tdT-mice and C57BL/6-mice were purchased from The Jackson Laboratory and maintained and cross-bred at our animal facility. PF4-Cre/Ai6(RCL-ZsGreen)-mice were bred at IRCCS San Raffaele Scientific Institute. PF4-Cre/MYH9 fl/fl -mice were a gift of Dr. Gachet (Le ´on et al., 2007) . aIIbÀ/À mice were a gift of Dr. Frampton (Emambokus and Frampton, 2003) . GPIb-hIl4R-mice were a gift of Dr. Ware (Kanaji et al., 2002) . All strains were backcrossed to and maintained on C57BL/6-background. If not otherwise stated, animals of same sex and age were randomly assigned to experimental groups. For sepsis experiments, knockout and control animals were paired according to age, sex and weight. All procedures performed on mice were approved by the local legislation on protection of animals (Regierung von Oberbayern, Munich).

Gaertner F., Ahmad Z., Rosenberger G., Fan S., Nicolai L., Busch B., Yavuz G., Luckner M., Ishikawa-Ankerhold H., Hennel R., Benechet A., Lorenz M., Chandraratne S., Schubert I., Helmer S., Striednig B., Stark K., Janko M., Böttcher R.T., Verschoor A., Leon C., Gachet C., Gudermann T., Mederos Y Schnitzler M., Pincus Z., Iannacone M., Haas R., Wanner G., Lauber K., Sixt M, & Massberg S. (2017). Migrating Platelets Are Mechano-scavengers that Collect and Bundle Bacteria. Cell, 171(6).

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