The architecture of the macroporous hydrogel was investigated using a scanning electron microscope (SEM) (FEI FEG-ESEM Quanta 600). After quick-freezing and lyophilization, each gel was immersed in liquid nitrogen and halved using two forceps. The generated fracture surface was sputtered with gold and platinum to make the sample conductive. SEM images were taken using high vacuum, at an accelerating voltage of 4-5 kV, a working distance of 6.2-7.4 mm and a spot size of 4.0.
Quanta 600 feg esem
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Quanta 600 FEG ESEM is a field emission scanning electron microscope (FE-SEM) that operates under low vacuum conditions. It is designed to provide high-resolution imaging and analysis capabilities for a wide range of materials and samples.
Automatically generated - may contain errors
Lab products found in correlation
Amicus esca 3400, by Shimadzu (1 mentions)
Tecnai g2 spirit twin, by Thermo Fisher Scientific (1 mentions)
Nicolet 6700 ft ir spectrometer, by Thermo Fisher Scientific (1 mentions)
Titan 80 300, by Thermo Fisher Scientific (1 mentions)
D8 advance, by Bruker (1 mentions)
Sputter coater 108, by Cressington (1 mentions)
5 protocols using quanta 600 feg esem
1
Investigating Macroporous Hydrogel Architecture
2
Backscattered Electron Imaging of Bone Mineral
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Backscattered electron (BSE) imaging was performed to investigate bone morphology and mineral content (brighter grey corresponds to higher mineral content) on regions with cortical lesions. Regions of interest were identified using high-resolution microCT. Controlled angle serial sectioning was performed to expose the PMMA block surface. BSE images were obtained using an environmental scanning electron microscope (FEI FEG-ESEM Quanta 600, FEI Company, Hillsboro, USA). This microscope operates at low vacuum (0.75 Torr), an accelerating voltage of 12.5 kV, a working distance of 9.9 mm, and a spot size of 4.0 [14 (link)]. Images taken at 100x magnification were merged together. Imaging conditions followed established quantitative backscattered electron imaging protocols for measurement of the mineral density distribution in human bone biopsies [15 (link)].
3
Comprehensive Materials Characterization with Advanced Microscopy and Spectroscopy
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Scanning electron microscopy (SEM) images were collected using FEI Quanta 600 FEG ESEM operated at 15 kV. Transmission electron microscopy (TEM) images were acquired using FEI Tecnai G2 Spirit Twin operated at 120 kV. High-resolution TEM (HRTEM), scanning TEM (STEM), and electron energy loss spectroscopy (EELS) were performed by FEI Titan 80-300 equipped with a field emission gun and spherical aberration corrector operated at 300 kV. EELS mapping was collected using a post-column filter in diffraction mode. X-ray photoelectron spectroscopy (XPS) was operated using Kratos Analytical AMICUS/ESCA 3400 equipped with an Mg-anode Kα excitation x-ray source (hν = 1253.6 eV) at 10 kV, 10 mA, and 2×10−6 Pa. The measured binding energies were calibrated based on C 1 s binding energy at 284.8 eV. X-ray powder diffraction (XRD) was carried out using the Bruker D8 Advance with a Cu Kα radiation. Fourier transform infrared (FTIR) spectroscopy was performed using the Thermo Scientific Nicolet 6700 FTIR spectrometer.
4
Microscopic Analysis of BMFC Anode Fibers
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Small clusters of carbon fibers from the BMFC anode that had been stored in fixative were observed under bright field microscopy at 100x, a few days after recovery, as an exploratory procedure. In these initial images, filamentous structures were visible and often appeared to span between and across fibers (Figure S1 ) prompting further investigation. Later, other fiber clusters from the 10 sampling locations were dehydrated in a graded series of ethanol from 10 to 100%. Specimens were mounted on aluminum SEM stubs with double-sided carbon tape, critical-point dried using an EMS 850 Critical Point Dryer, and sputter-coated with gold and palladium using a Cressington 108 sputter coater. The resultant specimens were observed under a FEI Quanta 600FEG ESEM at 5–10 kV. This instrument also provided elemental spectra by X-Ray Energy Dispersive Spectrometry (EDS).
5
Nanoscale Analysis of Electrospun Fibers
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