After extraction, proteins in cell lysates were first resolved by SDS–PAGE, and then transferred to nitrocellulose membrane and subsequently incubated with the primary antibody. The antibodies to HIF-1α (36169; 1:1000), pro-IL-1β (12242; 1:1000), Cleaved IL-1β (83186; 1:1000), Caspase-3 (9662; 1:1000), PARP (9542s; 1:1000) were obtained from Cell Signaling Technology. The antibody to Caspase-1 (AG-20B-0042, 1:1000) was obtained from Adipogen. The antibody to NLRP3(IMG-6668A; 1:1000) was obtained from Novus. The antibody to α-Tubulin (11224-1-AP, 1:3000) was obtained from Proteintech. After incubation with Horseradish peroxidase-conjugated secondary antibodies, the signals were visualized by enhanced chemiluminescence (Pierce, Rockford, IL, USA, 32106) according to the manufacturer's instruction. The relative band intensity was quantified using the ImageJ Analyzer software (Media Cybernetics, Bethesda, MD, USA).
Hif 1α 36169
Manufactured by Cell Signaling Technology
Sourced in United States
HIF-1α (36169) is a primary antibody that recognizes the hypoxia-inducible factor 1-alpha (HIF-1α) protein. HIF-1α is a subunit of the HIF-1 transcription factor, which plays a crucial role in the cellular response to low oxygen conditions (hypoxia).
Automatically generated - may contain errors
Lab products found in correlation
Caspase 3 9662, by Cell Signaling Technology (1 mentions)
Parp 9542, by Cell Signaling Technology (1 mentions)
Cleaved il 1β 83186, by Cell Signaling Technology (1 mentions)
Mitosox, by Thermo Fisher Scientific (1 mentions)
Mitotracker, by Thermo Fisher Scientific (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Transgene (1 mentions)
Cck 8 assay kit, by Meilun (1 mentions)
2 protocols using hif 1α 36169
1
Western Blot Analysis of Inflammatory Markers
2
Comprehensive Cellular Analysis Protocol
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The antibodies to CISD2 (60758 S), IRP2 (37135S), and HIF-1α (#36169) were obtained from Cell Signaling Technology (Danvers, MA, USA). The antibodies to Transferrin Receptor (sc-32272) were obtained from Santa Cruz (Dallas, TX, USA). The antibodies to β-Actin (ab8226) were obtained from Abcam (Cambridge, MA, USA). CCK-8 Assay Kit was obtained from Meilunbio (Dalian, China). C11-BODIPY (581/591), deferoxamine (DFO), Rhodamine B-[(1, 10-phenanthroline-5-yl)-aminocarbonyl] benzyl ester (RPA), DCF-DA, MitoTracker, and MitoSOX were obtained from Thermo Fisher Scientific (Waltham, MA, USA). The BeyoClick™ EdU Cell Proliferation Kit and ATP detection kit purchased from Beyotime (Shanghai, China), Annexin V-FITC/PI apoptosis detection kit was purchased from TransGen Biotech (Beijing, China).
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