Anti cd64 clone x54 5 7.1

Manufactured by BioLegend

Anti-CD64 (clone X54-5/7.1) is a monoclonal antibody that binds to the CD64 antigen. CD64 is a high-affinity Fc gamma receptor expressed on the surface of monocytes, macrophages, and neutrophils. This antibody can be used for the identification and characterization of cells expressing CD64.

Automatically generated - may contain errors

Lab products found in correlation

Facscalibur, by BD (1 mentions) Precision count beads, by BioLegend (1 mentions) Zombie nir fixable viability kit, by BioLegend (1 mentions) Anti iaie clone m5 114, by BioLegend (1 mentions) Anti ly6g clone 1a8, by BioLegend (1 mentions) Anti f4 80 clone bm8, by BioLegend (1 mentions) Anti cd11b clone m1 70, by Thermo Fisher Scientific (1 mentions) Anti ly6c clone hk1, by BioLegend (1 mentions) Collagenase type 2, by Worthington (1 mentions) Dnase, by Merck Group (1 mentions) Anti cd45.2 clone 104, by Thermo Fisher Scientific (1 mentions) Anti cd45.1 clone a20, by BD (1 mentions) Anti cd31 clone mec13, by BD (1 mentions) Anti cd11b clone m1 70, by BioLegend (1 mentions)

Spelling variants of this product

CD64 (X54-5/7.1, (16 citations) CD64 (clone X54-5/7.1, (8 citations) X54-5/7.1 (8 citations) Anti-CD64 (6 citations) Anti-CD64 (X54–5/7.1, (3 citations) Clone X54–5/7.1 (3 citations)

2 protocols using anti cd64 clone x54 5 7.1

Murine Peritoneal Cell Profiling

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Mice were euthanized with CO₂ as described above. Peritoneal exudate was taken from four CLP + Vehicle and five CLP + RvE1 mice. Peritoneal cells were differentiated using anti-CD11b (clone M1/70; eBioscience), anti-F4/80 (clone BM8; BioLegend), anti-Ly6G (clone 1A8; BioLegend), anti-I-A/I-E (clone M5/114.15.2; BioLegend), anti-Ly6C (clone HK1.4; BioLegend), anti-CD64 (clone X54-5/7.1; BioLegend) antibodies. Zombie NIR™ Fixable Viability Kit (Biolegend) was used to identify live cells. Cell counts were determined using Precision Count Beads (Biolegend). Ten thousand live cell events were acquired with a FACSCalibur (Becton Dickinson) and analyzed using FlowJo analysis software (version 9.2, Treestar Inc.).

Chen J., Purvis G.S., Collotta D., Al Zoubi S., Sugimoto M.A., Cacace A., Martin L., Colas R.A., Collino M., Dalli J, & Thiemermann C. (2020). RvE1 Attenuates Polymicrobial Sepsis-Induced Cardiac Dysfunction and Enhances Bacterial Clearance. Frontiers in Immunology, 11, 2080.

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Isolation and Characterization of Cardiac Cells

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Heart tissue was cut into small pieces and digested by placement into a RPMI 1640 solution containing Type 2 collagenase (1 mg/ml) (Worthington Biochemical Corporation) and 10 U/ml DNase (Sigma-Aldrich) at 37°C for 60 minutes. The digested tissue was then passed through a 70-μm cell strainer and treated with ACK lysing buffer. Cells were stained with fluorochrome-labeled anti-CD45.2 (clone 104, eBioscience), anti-CD45.1 (clone A20, BD Biosciences), anti-MHC class II (clone M5/114.15.2, BioLegend), anti CD11b (clone M1/70, BioLegend), anti-CCR2 (clone 575301, R&D Systems), anti-CD64 (clone X54-5/7.1, BioLegend), and anti-CD31 (clone MEC13.3, BD Biosciences).

Li W., Hsiao H.M., Higashikubo R., Saunders B.T., Bharat A., Goldstein D.R., Krupnick A.S., Gelman A.E., Lavine K.J, & Kreisel D. (2016). Heart-resident CCR2+ macrophages promote neutrophil extravasation through TLR9/MyD88/CXCL5 signaling. JCI Insight, 1(12), e87315.

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