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Fmoc l histidine amino acids

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Fmoc-L-histidine amino acids are laboratory reagents used in the synthesis and analysis of peptides and proteins. They serve as building blocks for the construction of specific amino acid sequences. The Fmoc (fluorenylmethyloxycarbonyl) protecting group allows for controlled addition of the histidine residue during peptide synthesis.

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Lab products found in correlation

Chemiluminescence, by GE Healthcare (2 mentions) H3k4me2, by Cell Signaling Technology (2 mentions) Setd3, by Abcam (2 mentions) Pan actin, by Cytoskeleton (2 mentions) β tubulin, by Merck Group (2 mentions) H3k36me2, by Cell Signaling Technology (2 mentions) Alexa488 anti rabbit antibody, by Thermo Fisher Scientific (2 mentions) Anti rabbit and anti mouse peroxidase conjugated antibodies, by Jackson ImmunoResearch (2 mentions)

2 protocols using fmoc l histidine amino acids

1

Biotinylated Peptide Synthesis and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Biotinylated peptides were synthesized at the UNC High-Throughput
Peptide Synthesis and Array Facility (Chapel Hill, NC)22 (link). Fmoc-L-histidine amino acids with
either (1-me) or (3-me) modifications (Sigma-Aldrich) were used in the synthesis
of these peptides and synthesized peptides were purified by HPLC (>92%
purity). Antibodies used in this study: SETD3 (Abcam), pan-actin (Cytoskeleton),
beta-tubulin (Millipore), H3K4me2 (Cell Signaling), H3K36me2 (Cell Signaling),
H73(3-me) see below, GFP (Invitrogen), Alexa488 anti-rabbit antibody (Life
Technologies), anti-rabbit and anti-mouse peroxidase conjugated antibodies
(Jackson Immunoresearch). Western blots were visualized by chemiluminescence (GE
Healthcare).
Wilkinson A.W., Diep J., Dai S., Liu S., Ooi Y.S., Song D., Li T.M., Horton J.R., Zhang X., Liu C., Trivedi D.V., Ruppel K.M., Vilches-Moure J.G., Casey K.M., Mak J., Cowan T., Elias J.E., Nagamine C.M., Spudich J.A., Cheng X., Carette J.E, & Gozani O. (2018). SETD3 is an Actin Histidine Methyltransferase that Prevents Primary Dystocia. Nature, 565(7739), 372-376.
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2

Biotinylated Peptide Synthesis and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Biotinylated peptides were synthesized at the UNC High-Throughput
Peptide Synthesis and Array Facility (Chapel Hill, NC)22 (link). Fmoc-L-histidine amino acids with
either (1-me) or (3-me) modifications (Sigma-Aldrich) were used in the synthesis
of these peptides and synthesized peptides were purified by HPLC (>92%
purity). Antibodies used in this study: SETD3 (Abcam), pan-actin (Cytoskeleton),
beta-tubulin (Millipore), H3K4me2 (Cell Signaling), H3K36me2 (Cell Signaling),
H73(3-me) see below, GFP (Invitrogen), Alexa488 anti-rabbit antibody (Life
Technologies), anti-rabbit and anti-mouse peroxidase conjugated antibodies
(Jackson Immunoresearch). Western blots were visualized by chemiluminescence (GE
Healthcare).
Wilkinson A.W., Diep J., Dai S., Liu S., Ooi Y.S., Song D., Li T.M., Horton J.R., Zhang X., Liu C., Trivedi D.V., Ruppel K.M., Vilches-Moure J.G., Casey K.M., Mak J., Cowan T., Elias J.E., Nagamine C.M., Spudich J.A., Cheng X., Carette J.E, & Gozani O. (2018). SETD3 is an Actin Histidine Methyltransferase that Prevents Primary Dystocia. Nature, 565(7739), 372-376.
+ Open protocol
+ Expand

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