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Horseradish peroxidase linked anti rabbit igg antibody

Manufactured by Santa Cruz Biotechnology

Horseradish peroxidase-linked anti-rabbit IgG antibody is a secondary antibody used in various immunoassay techniques, such as Western blotting and ELISA. It is designed to detect and bind to rabbit primary antibodies, with the horseradish peroxidase enzyme serving as a reporter molecule for signal detection.

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2 protocols using horseradish peroxidase linked anti rabbit igg antibody

1

Quantifying Protein Expression in Cardiac Cells

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Rabbit polyclonal antibody to cystathionine β-synthase (sc-67154, CBS), mouse monoclonal antibody to cystathionine γ-lyase (sc-374249, CSE) and horseradish peroxidase-linked anti-rabbit IgG antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Monoclonal antibodies against MMP-9 (AB 19016; rabbit), NMDA-R1 (AB 9864, rabbit) and GAPDH (ABS 16, rabbit) were purchased from Millipore (Temecula, CA). Rabbit polyclonal antibody to connexin-40 (36-4900, Cx-40) and mouse monoclonal antibody to connexin-43 (35-5000, Cx-43) were purchased from Life Technologies (Grand Island, NY). Sodium hydrosulfide hydrate (NaHS; Cat # 161527) was from Sigma chemicals (St. Louis, MO). Polyvinylidene fluoride (PVDF) membrane was from Bio-Rad (Hercules, CA). All other chemicals used in this study were of analytical grade.
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2

Antibody and Reagent Sourcing for MMP-Collagen Study

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Rabbit polyclonal antibodies against matrix metalloproteinases-9, -13, -14 and Collagen-4a were from Abcam (Cambridge, UK). Collagen-1a from Novus Biologicals (Littleton, CO). Horseradish peroxidase-linked anti-rabbit IgG antibody was from Santa Cruz Biotechnology (Santa Cruz, CA). Poly ADP ribose polymerase 1 (PARP1) and anti-GAPDH antibody were purchased from Millipore (Temecula, CA). GYY4137 was from Sigma. PVDF membrane was from Bio-Rad (Hercules, CA). Dihydroethidium (DHE) was purchased from Invitrogen (Carlsbad, CA). All other analytical reagents were from Sigma-Aldrich (Saint Louis, MO) or available highest grade.
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