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Re 595

Manufactured by Merck Group
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Sourced in Germany

The Re 595 is a laboratory equipment product manufactured by Merck Group. It is designed to perform a core function, but a detailed and unbiased description cannot be provided without the risk of extrapolation or interpretation.

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Lab products found in correlation

Phrodo green e coli bioparticles conjugate for phagocytosis, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Nacalai Tesque (1 mentions) Brefeldin a, by BioLegend (1 mentions)

4 protocols using re 595

1

LPS-Induced Splenic DC Subset Analysis

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All mice were housed under specific pathogen-free conditions in the Centenary Institute Animal Facility. [C57BL/6× B10.BR]F1 mice on a CD45.1/CD45.2 heterozygous background were used for all experiments. Our unpublished results have identified no differences in phenotype and function of splenic DC subsets isolated from [C57BL/6× B10.BR]F1 mice and their C57BL/6 counterparts. Mice were injected intraperitoneally with 25 μg LPS (rough strains from Salmonella enterica serotype Minnesota Re 595, Sigma-Aldrich) per mouse. Animals were sacrificed mice 24 hours after LPS injection.
Hancock D.G., Shklovskaya E., Guy T.V., Falsafi R., Fjell C.D., Ritchie W., Hancock R.E, & Fazekas de St Groth B. (2014). A Systems Biology Approach to the Analysis of Subset-Specific Responses to Lipopolysaccharide in Dendritic Cells. PLoS ONE, 9(6), e100613.
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2

Proliferation Assays with EdU

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The 5-ethynyl-2ʹ-deoxyuridine (EdU) used in the in vitro and in vivo proliferation assays was purchased from Tokyo Chemical Industry Co. (Tokyo, Japan). RPMI1640 was purchased from Nacalai Tesque. Lipid A purified from Salmonella minnesota (Re-595) was purchased from Sigma–Aldrich (Merck, Darmstadt, Germany). Brefeldin A was purchased from BioLegend (San Diego, CA, USA). pHrodo Green E. coli bioparticles Conjugate for Phagocytosis was purchased from Invitrogen.
Hiranuma R., Sato R., Yamaguchi K., Nakamizo S., Asano K., Shibata T., Fukui R., Furukawa Y., Kabashima K, & Miyake K. (2023). Aberrant monocytopoiesis drives granuloma development in sarcoidosis. International Immunology, 36(4), 183-196.
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3

Intravenous MPLA Administration in Sheep

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MPLA derived from Salmonella enterica serotype Minnesota Re 595 was purchased from Sigma-Aldrich Co. (Catalog #: L6895, St. Louis, MO), solubilized in sterile water containing 0.2% triethylamine solution (1 mg/ml) and sonicated for 1 hour at 40°C. For administration, MPLA was diluted in saline solution (25 ml) and administered by intravenous infusion (2.5 μg/kg) over 50 minutes. Physiologic measurements were performed prior to and at 5, 10, 20, 30, 45, 60, 75, 90, 105, and 120 minutes after initiation of MPLA infusion in awake sheep. During this and previous (burn induction) phases, sheep were fluid resuscitated with LR using our standard formula [29 (link)].
Fukuda S., Ihara K., Bohannon J.K., Hernandez A., Patil N.K., Luan L., Stothers C., Stark R., Prough D.S., Herndon D.N., Sherwood E.R, & Enkhbaatar P. (2020). Monophosphoryl Lipid A Attenuates Multiorgan Dysfunction during Post-burn Pseudomonas aeruginosa Pneumonia in Sheep. Shock (Augusta, Ga.), 53(3), 307-316.
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4

MPLA-Induced Immune Activation Protocol

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MPLA derived from Salmonella enterica serotype Minnesota Re 595, was purchased from Sigma-Aldrich Corp. (St. Louis, MO) or Invivogen. MPLA was solubilized in 0.2% triethylamine solution (1 mg/ml) or DMSO and sonicated for 1 hour at 40°C. For treatment, MPLA was diluted in LR solution(100 μg/ml) and then administered by i.v. or i.p. injection at 20μg in 0.2 ml. Vehicle treated mice received i.v. or i.p. injection of 0.2 ml LR solution.
Fensterheim B.A., Young J.D., Luan L., Kleinbard R.R., Stothers C.L., Patil N.K., McAtee-Pereira A.G., Guo Y., Trenary I., Hernandez A., Fults J.B., Williams D.L., Sherwood E.R, & Bohannon J.K. (2018). The TLR4 agonist monophosphoryl lipid A drives broad resistance to infection via dynamic reprogramming of macrophage metabolism. Journal of immunology (Baltimore, Md. : 1950), 200(11), 3777-3789.
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