Vidas cdab assay

Manufactured by bioMérieux

Sourced in France

The Vidas CDAB assay is a laboratory equipment product developed by bioMérieux. It is designed to detect and quantify Clostridium difficile antigens in stool samples. The assay utilizes enzyme-linked fluorescent assay (ELFA) technology to provide automated and standardized testing for the diagnosis of Clostridium difficile infection.

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Lab products found in correlation

Illumigene c difficile, by Meridian Bioscience (1 mentions)

Spelling variants of this product

Vidas assay (4 citations)

2 protocols using vidas cdab assay

Routine Detection of C. difficile

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C. difficile was identified using the routine method put in place in the laboratory, which initially was the detection of TcdA and TcdB on faecal specimens using an enzyme-linked ﬂuorescent immunoassay (ELFA, Vidas CDAB assay, bioMerieux, Marcy-l’Étoile, France) and from June 2011 by Illumigene™ C. difficile (MeridianBioscience Inc., Cincinnati, OH), both performed according to the manufacturers’ instructions. All C. difficile positive specimens were cultured anaerobically on cycloserine-cefoxitin-fructose-agar for 48 h and retained C. difficile isolates were stored at −80°C.

Magnusson C., Mernelius S., Bengnér M., Norén T., Serrander L., Forshell S, & Matussek A. (2022). Characterization of a Clostridioides difficile outbreak caused by PCR ribotype 046, associated with increased mortality. Emerging Microbes & Infections, 11(1), 850-859.

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C. difficile Identification Protocol

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Ethical approval was not required because this study was for clinical laboratory testing that was not subject to human subjects review. We designed a protocol for culturing C. difficile in CDIF medium without pretreating the specimen, followed by Gram staining and PRO disc (PRO disc K1532B, Key Scientific Products, Round Rock, TX, USA) testing [10 (link)] for the identification of C. difficile in colonies produced on CDIF agar. To confirm the utility of this protocol, we prospectively collected a total of 1,402 stool specimens from patients with suspected C. difficile infection from November 2011 to March 2012 at Samsung Medical Center in Seoul, Korea. On arrival at the clinical microbiologic laboratory, these specimens were immediately tested by using the C. difficile toxin A & B immunoassay (CDAB; Vidas CDAB assay, bioMérieux, Marcy-l'Etoile, France) and inoculated into CDIF. In addition to Gram staining and PRO disc testing, the cultured isolates were subjected to automated analysis by using the Vitek 2 ANC kit (bioMérieux) and 16S rDNA sequencing for the identification of C. difficile. Fig. 1 presents a schematic overall process for confirmation of our protocol.

Park K.S., Ki C.S, & Lee N.Y. (2015). Isolation and Identification of Clostridium difficile Using ChromID C. difficile Medium Combined With Gram Staining and PRO Disc Testing: A Proposal for a Simple Culture Process. Annals of Laboratory Medicine, 35(4), 404-409.

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