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28 protocols using chromatographic grade acetonitrile

1

Graphite-Based Phthalate Analysis Protocol

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Graphite powder, KMnO4, NaNO3, H2SO4, HCl, DMF, N,N-dicyclohexylcarbodiimide (DCC), N-ethyl-N-(3-(dimethylamino) propyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). The ionic liquids 1-aminopropyl-3-methylimidazolium bromide [APMIM][Br], 1-aminoethyl-3-methylimidazolium bromide [AEMIM][Br], 1-aminopropyl-3-imidazolium bis(trifluoromethylsulfonyl)imine [APMIM][NTf(2)] and 1-aminoethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide [AEMIM][NTf(2)] were purchased from the Lanzhou Institute of Chemical Physics (Lanzhou, China). The standard PAE samples dimethyl phthalate (DMP), diethyl phthalate (DEP), dipropyl phthalate (DPrP), dibutyl phthalate (DBP), diisobutyl phthalate (DIBP), di(2-ethylhexyl) phthalate (DEHP), di(n-pentyl) phthalate (DNPP), di(n-octyl) phthalate (DNOP) and benzylbutyl phthalate (BBP) were purchased from ANPEL Laboratory Technologies Incorporation (Shanghai, China). Chromatographic grade acetonitrile, methanol and isopropanol were purchased from Sigma-Aldrich (St. Louis, MO). All the aqueous solutions were prepared using ultrapure water (18.2 MΩ/cm).
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2

Aflatoxin B1 Analysis Protocol

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AFB1 (2, 3, 6α, 9α-tetrahydro-4-methoxycyclopenta [c] furo [2, 3:45] furo [2, 3-h] chromene-1, 11-dione; purity >98%) was obtained from Sigma (Sigma-Aldrich, St. Louis, MO, USA). Methyl alcohol, dimethyl sulfoxide (DMSO), and other reagents are analytical reagents and purchased from Sinopharm (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) Peanut oil was purchased from Wal-Mart supermarket in Wuhan, China.
For high-performance liquid chromatography (HPLC) and TQEF-MS/MS analysis, chromatographic grade acetonitrile and Methyl alcohol were purchased from Sigma (St. Louis, MO, USA). The deionized water was obtained from a Milli-Q SP Reagent Water system (Millipore, Bedford, MA, USA).
For cytotoxicity tests, human embryo hepatocytes (L-02 cells) were purchased from Procell (Procell Co. Ltd., Wuhan, China). Culture medium RPMI-1640 and fetal calf serum were purchased from HyClone ((GE-Healthcare, Logan, UT, USA). The 0.05% trypsin solution was from Thermo Fisher (Thermo Fisher Scientific, Waltham, MA, USA).
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3

Mogroside V Isolation and Characterization

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Mogroside V with a purity of 98% was purchased from Nanjing Spring & Autumn Biological Engineering Co., Ltd. (Nanjing, China). Chromatographic-grade acetonitrile and phosphoric acid were purchased from Sigma-Aldrich (St. Louis, Mo.). Diphenoxylate tablets were purchased from Changzhou Green Peak Environmental Protection Tech (50 mg/tablet, Changzhou, China). Mosapride citrate dispersible tablets were purchased from Chengdu Kanghong Pharmaceutical Group Co., Ltd. (5 mg/tablet, Chengdu, China). Activated charcoal was supplied by Chengdu Changzheng Glass Co., Ltd. (Chengdu, China). The mouse motilin (MTL) ELISA kit was supplied by Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China). The other reagents were of analytical grade and commercially available.
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4

Cypermethrin Degradation by Microbial Cultures

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Cypermethrin (97%) was purchased from Jiangsu Yangnong Chemical Group Co., Ltd, 3-PBA (98%) and chromatographic grade acetonitrile were purchased from Sigma-Aldrich Chemical Co. (Shanghai, China). All other chemicals and solvents used in this study were analytical grade. Cypermethrin was dissolved in methanol as stock solutions (24 mM), and sterilized by membrane filtration (0.22 μm). The R2A medium consisted of 0.25 g/L tryptone, 0.5 g/L yeast extract and 0.5 g/L casein acid hydrolyzate. The Luria-Bertani (LB) medium consisted of 10 g/L tryptone, 5 g/L yeast extract, and 10 g/L NaCl, pH was adjusted to 7.0. Mineral salt medium (MSM) consisted of 1.0 g/L NH4Cl, 1.0 g/L NaCl, 1.5 g/L K2HPO4, 0.5 g/L KH2PO4 and 0.2 g/L MgSO4⋅7H2O, pH 7.0. For solid medium, 1.5% of agar was added. All medium was autoclaved at 121°C for 30 min.
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5

Enzymatic Seafood Protein Extraction

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Round scad was purchased from the Vanguard Supermarket (Guangzhou, China). Neutrase, protamex and alcalase were purchased from Cool Chemical Technology Co., Ltd. (Beijing, China). Cytochrome C (12,400 Da), aprotinin (6511.44 Da), bacitracin (1422.69 Da), L-glutathione oxidized (612.63 Da) and glutathione reduced (307.32 Da) were obtained from Macklin Biochemical Technology Co., Ltd. (Beijing, China). Xanthine, xanthine oxidase and chromatographic grade acetonitrile were purchased from Sigma Chemical Co. (St. Louis, MO, USA). All other chemicals and reagents were of analytical grade and purchased from Beiluo Biological Technology Co., Ltd. (Beijing, China).
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6

Metabolic Profiling of L. bulgaricus ND02

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The L. bulgaricus ND02 strain used in the experiment was obtained from the LAB Culture Collection, Inner Mongolia Agricultural University, China. Skim milk powder (protein 30%) was purchased from Fonterra Co. Ltd. (NZMP Ltd.). The Acquity UPLC-Xevo-G2-QTOF-MS system, MassLynx 4.1 Workstation, Progenesis QI, and the leucine-enkephalin standard were purchased from Waters Corp. Chromatographic grade acetonitrile, ammonium hydroxide solution, and formic acid were purchased from Sigma Aldrich.
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7

Analytical-Grade Chemical Characterization

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All the chem i cals used in this study were of an a lyt i cal grade and were used with out fur ther pu rifi ca tion. LVN (pu rity 98%) was obtained from Sigma Aldrich. Hep tahy drate fer rous sul fate (pu rity 99%), an hy drous sodium sul fate (pu rity 99%), sul fu ric acid and sodium hy drox ide were sup plied from J.T Baker and Fer mont. Chromato graphic grade ace toni trile and formic acid were pur chased from Sigma-Aldrich. Syn thetic so lu tions were pre pared with deion ized water.
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8

Chromatographic Analysis of n-Alkanes

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The hexane was acquired from Fisher Scientific (FL, NJ, USA). Calcium chloride dihydrate, sodium chloride, pyridine and EDTA were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Chromatographic-grade acetonitrile, acetic acid and methanol were purchased from Merck (Darmstadt, Germany). N-alkanes (C8-C20) and all standards were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China) and Sigma-Aldrich (St. Louis, MO, USA).
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9

Chicoric Acid Transport in Caco-2 Cells

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Chicoric acid (purity ≥98%) was purchased from Mansite Biological Technology Co., Ltd. (Chengdu, China). The Caco-2 cell line was obtained from National Experimental Cell Resource Sharing Platform (Peking, China). HBSS, DMEM, FBS and other cell culture reagents were all obtained from Gibco, Thermo Fisher Scientific. Heparin sodium injection (6250 IU/mL) was purchased from Tianjin Biochem Pharmaceutical Co. Ltd. (Tinjin, China). Chromatographic grade acetonitrile and methanol were obtained from EMD Millipore Corporation (Billerica MA, USA). Analytical grade hydrochloric acid, phosphoric acid and other chemicals were obtained from Tianjin Concord Chemical Reagent Company (Tianjin, China). Ultrapure water of Milli-Q grade was used throughout the study.
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10

CPP Mixtures: Characterization and Applications

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Infinitus (China) Company Ltd. (Guangzhou, China) donated two kinds of commercially available CPP mixtures (CPP1 and CPP2) for use in these experiments. Nitric acid and chromatographic-grade acetonitrile were bought from Merck (Merck KGaA, Darmstadt, Genmany). Cell culture media, penicillin, streptomycin, non-essential amino acids, Hank's balanced salt solution (HBSS) without Ca2+ and Mg2+, and fetal bovine serum were procured from Sigma (St. Louis, MO, USA). All other reagents were of an analytical grade.
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