Ifn λ

Manufactured by R&D Systems

Sourced in United States

IFN-λ is a recombinant human protein that belongs to the type III interferon family. It is involved in the innate immune response. The core function of IFN-λ is to induce the expression of interferon-stimulated genes.

Automatically generated - may contain errors

Lab products found in correlation

Ifn α, by Thermo Fisher Scientific (4 mentions) Ifn α, by R&D Systems (2 mentions) X vivo 15, by Lonza (2 mentions) Human serum albumin (hsa), by Bayer (2 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (2 mentions) Ifn β, by PBL Assay Science (2 mentions) Interleukin 3 (il 3), by R&D Systems (1 mentions) Ifn α, by PBL Assay Science (1 mentions) Tumor necrosis factor (tnf), by BioLegend (1 mentions) Cxcl12, by R&D Systems (1 mentions) Interleukin 6 (il 6), by BioLegend (1 mentions) Ht1080, by American Type Culture Collection (1 mentions) Human ifn γ, by R&D Systems (1 mentions) Msc2530818, by Selleck Chemicals (1 mentions) Hek293t, by American Type Culture Collection (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Ifn β, by Thermo Fisher Scientific (1 mentions) Il 12p40, by BD (1 mentions)

Spelling variants of this product

Recombinant murine IFN-λ (2 citations)

11 protocols using ifn λ

Quantification of Secreted Cytokines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse: Secreted CXCL12 (R&D systems), IFNλ (R&D systems), and IFNα (R&D systems) were measured by ELISA according to the manufacturer’s instructions. Human: Secreted CXCL12 (R&D Systems), IL-3 (R&D Systems), IL-6 (Biolegend), TNF (Biolegend), IFNλ (R&D systems), and IFNα (PBL assay science, Piscataway, NJ, USA) were measured by ELISA according to the manufacturer’s instructions.

Bénard A., Jacobsen A., Brunner M., Krautz C., Klösch B., Swierzy I., Naschberger E., Podolska M.J., Kouhestani D., David P., Birkholz T., Castellanos I., Trufa D., Sirbu H., Vetter M., Kremer A.E., Hildner K., Hecker A., Edinger F., Tenbusch M., Mühl-Zürbes P., Steinkasserer A., Richter E., Streeck H., Berger M.M., Brenner T., Weigand M.A., Swirski F.K., Schett G., Grützmann R, & Weber G.F. (2021). Interleukin-3 is a predictive marker for severity and outcome during SARS-CoV-2 infections. Nature Communications, 12, 1112.

+ Open protocol

+ Expand

Characterization of Cell Lines and Reagents

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293T, A549, HeLa, MEF, Vero, and HT1080 cells were obtained from the American Type Culture Collection. Lats1/2^−/− MEF cells were provided by L. Chen (Xiamen University, China). 2fTGH and U3A cells were described previously (21 (link)). B16 melanoma cells were gifts from L. Hu (Soochow University, China). Following procedures previously described (21 (link)), we cultured all cells at 37°C under 5% CO₂ in Dulbecco’s modified Eagle’s medium (DMEM; HyClone) supplemented with 10% fetal bovine serum (FBS; Gibco, Life Technologies), penicillin (100 U/ml), and streptomycin (100 μg/ml). Recombinant human IFN-ɑ was from PBL Interferon Source. Recombinant mIFNβ, human IFN-γ, and IFN-λ were purchased from R&D Systems. Recombinant Tyk2 proteins were purchased from Abcam. The CDK8 inhibitor MSC2530818 was purchased from Selleck. Non–phospho-peptide (NP-Pep) and phospho-peptide (P-Pep) were from GL Biochem (Shanghai). Recombinant glutathione S-transferase (GST)–LATS1, Flag peptides (F3290), Puromycin, and other chemicals were purchased from Sigma-Aldrich. The polyclonal antibody to phosphorylated Ser⁶² of CDK8 was generated by GL Biochem (Shanghai).

Zuo Y., He J., Liu S., Xu Y., Liu J., Qiao C., Zang L., Sun W., Yuan Y., Zhang H., Chen X., Jin L., Miao Y., Huang F., Ren T., Wang J., Qian F., Zhu C., Zhang W., Liu Y., Xu G., Ma F, & Zheng H. (2022). LATS1 is a central signal transmitter for achieving full type-I interferon activity. Science Advances, 8(14), eabj3887.

+ Open protocol

+ Expand

Vaginal Viral Titer Quantification

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Vaginal fluids were collected on the indicated days by pipetting a volume of 50 μL of PBS in and out of the vagina 20 times. Viral titers were measured by titration of vaginal fluids on Vero cells for 72 h in duplicate as described previously37 (link). The levels of IFN-α (eBioscience, San Diego, CA) and IFN-λ (R & D systems, Minneapolis, MN) in vaginal fluids were measured by ELISA according to manufacturer’s instructions.

Oh J.E., Lee M.S., Kim Y.J, & Lee H.K. (2016). OASL1 deficiency promotes antiviral protection against genital herpes simplex virus type 2 infection by enhancing type I interferon production. Scientific Reports, 6, 19089.

+ Open protocol

+ Expand

Monocytic Dendritic Cell Differentiation Kinetics

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 4

In this example monocytic dendritic precursor cells that were cultured in the presence of a adhesion blocking agent were tested for the kinetics of in vitro differentiation into dendritic cells in medium supplemented with GM-CSF alone.

Briefly, cryopreserved monocytes were resuspended at a concentration of 1×10⁶cells/ml in DC culture media containing X-VIVO-15® (BioWhittaker), 2% human serum albumin (Bayer), and 500 U/ml GM-CSF (Immunex). The cell suspension was transferred into a Teflon® bag (Americal Fluoroseal), and cultured for 5 days in a 6% CO₂, 37° C. humidified incubator. On day 5, maturation agents (1:400 dilution of inactivated BCG (Organon-Teknika) and 500 Vlml IFNλ (R and D Systems) were added to the culture bag. The maturation event was allowed to proceed for about 18 hours. Cells were harvested daily from the bag for flow cytometric analyses of the expression of CD14 and CD1a. Data from these analyses demonstrated that the conversion from monocytes (CD14+ and CD1a⁻) to DCs (CD14⁻, CD1a⁺) started between 1 and 2 days after the start of the culture. (FIG. 5). By day 3, phenotype conversions were completed.

US10731130B2. Generation of dendritic cells from monocytic dendritic precursor cells with GM-CSF in the absence of additional cytokines (2020-08-04). Northwest Biotherapeutics, Inc. [US]. Inventors: Benjamin A. Tjoa [US], Marnix L. Bosch [US].

+ Open protocol

+ Expand

Differentiating Monocytic Dendritic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 4

Briefly, cryopreserved monocytes were resuspended at a concentration of 1×10⁶cells/ml in DC culture media containing X-VIVO-15® (BioWhittaker), 2% human serum albumin (Bayer), and 500 U/ml GM-CSF (Immunex). The cell suspension was transferred into a Teflon® bag (Americal Fluoroseal), and cultured for 5 days in a 6% CO₂, 37° C. humidified incubator. On day 5, maturation agents (1:400 dilution of inactivated BCG (Organon-Teknika) and 500 Vlml IFNλ (R and D Systems) were added to the culture bag. The maturation event was allowed to proceed for about 18 hours. Cells were harvested daily from the bag for flow cytometric analyses of the expression of CD14 and CD1a. Data from these analyses demonstrated that the conversion from monocytes (CD14⁺ and CD1a⁻) to DCs (CD14⁻, CD1a⁺) started between 1 and 2 days after the start of the culture. (FIG. 5). By day 3, phenotype conversions were completed.

US11827903B2. Generation of dendritic cells from monocytic dendritic precursor cells with GM-CSF in the absence of additional cytokines (2023-11-28). Northwest Biotherapeutics, Inc. [US]. Inventors: Benjamin A. Tjoa [US], Marnix L Bosch [US].

+ Open protocol

+ Expand

Innate Immune Response to HSV Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols

BM cells were isolated from the femurs and tibiae of mice, and single cells were prepared by passage through 70 μm cell strainers (SPL). 5 × 10⁵ or 1 × 10⁶ BM cells were stimulated with GFP HSV-1 or TK- HSV-2 at the indicated MOIs and cultured at 37 °C for 18 h. Levels of IFN-α, IFN-β (eBioscience), IFN-λ (R & D systems), and IL-12p40 (BD Biosciences, San Jose, CA) from supernatants were measured by ELISA. Cells were collected and stained for flow cytometric analysis.

+ Open protocol

+ Expand

Cytokine Production Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Levels of IFN-β and -λ and IL-6 production were assessed by ELISA after 24 h of cell stimulation. Separate ELISAs were performed for IFN-β (PBL), IFN-λ (R&D Biosystems), and IL-6 (eBioscience) according to the kit manufacturers’ instructions.

Chen J., Jing H., Martin-Nalda A., Bastard P., Rivière J.G., Liu Z., Colobran R., Lee D., Tung W., Manry J., Hasek M., Boucherit S., Lorenzo L., Rozenberg F., Aubart M., Abel L., Su H.C., Soler Palacin P., Casanova J.L, & Zhang S.Y. (2021). Inborn errors of TLR3- or MDA5-dependent type I IFN immunity in children with enterovirus rhombencephalitis. The Journal of Experimental Medicine, 218(12), e20211349.

+ Open protocol

+ Expand

Cytokine Quantification by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse: Secreted TNFα (Biolegend, San Diego, CA, USA), IL-10 (Biolegend), IL-1β (Biolegend), IFNλ (R&D systems), IFNβ (Biolegend) and IFNα (R&D systems) were measured by ELISA according to the manufacturer’s instructions. Human: Secreted IFNλ (R&D Systems), IFNγ (Biolegend), IL-10 (Biolegend) and TNFα (Biolegend) were measured by enzyme linked immunosorbent assay (ELISA) according to the manufacturer’s instructions. Quantification of human IL-3 (R&D Systems) was performed in combination with chemiluminescent detection (R&D Systems) for increased sensitivity. The assays were performed according to the manufacturer’s instructions and measured in a microplate reader set to luminescence mode (BMG Labtech, Ortenberg, Germany) with an integration time of 2 seconds per well, yielding a sensitivity of 3.9 pg/ml IL-3.

Bénard A., Hansen F.J., Uhle F., Klösch B., Czubayko F., Mittelstädt A., Jacobsen A., David P., Podolska M.J., Anthuber A., Swierzy I., Schaack D., Mühl-Zürbes P., Steinkasserer A., Weyand M., Weigand M.A., Brenner T., Krautz C., Grützmann R, & Weber G.F. (2023). Interleukin-3 protects against viral pneumonia in sepsis by enhancing plasmacytoid dendritic cell recruitment into the lungs and T cell priming. Frontiers in Immunology, 14, 1140630.

+ Open protocol

+ Expand

Cytokine Quantification in BAL

Check if the same lab product or an alternative is used in the 5 most similar protocols

ELISAs for IL-5 (R&D), IL-13 (R&D), IFNγ (ThermoFisher), IFNβ (R&D), and IFNλ (R&D) were performed on BAL supernatants according to the manufacturers' protocols. ELISA plates were read on a model 680 microplate reader (Bio-Rad) at 450 nm.

Cavagnero K.J., Badrani J.H., Naji L.H., Amadeo M.B., Leng A.S., Lacasa L.D., Strohm A.N., Renusch S.R., Gasparian S.S, & Doherty T.A. (2021). Cyclic-di-GMP Induces STING-Dependent ILC2 to ILC1 Shift During Innate Type 2 Lung Inflammation. Frontiers in Immunology, 12, 618807.

+ Open protocol

+ Expand

Quantifying Cytokine Production

Check if the same lab product or an alternative is used in the 5 most similar protocols

Levels of IFN-α, -β, and -λ and IL-6 production were assessed by ELISA after 24 h of cell stimulation. Separate ELISAs were performed for each of IFN-α (eBioscience), IFN-β (PBL), IFN-λ (R&D Biosystems), and IL-6 (eBioscience), according to the kit manufacturer’s instructions.

Lim H.K., Huang S.X., Chen J., Kerner G., Gilliaux O., Bastard P., Dobbs K., Hernandez N., Goudin N., Hasek M.L., García Reino E.J., Lafaille F.G., Lorenzo L., Luthra P., Kochetkov T., Bigio B., Boucherit S., Rozenberg F., Vedrinne C., Keller M.D., Itan Y., García-Sastre A., Celard M., Orange J.S., Ciancanelli M.J., Meyts I., Zhang Q., Abel L., Notarangelo L.D., Snoeck H.W., Casanova J.L, & Zhang S.Y. (2019). Severe influenza pneumonitis in children with inherited TLR3 deficiency. The Journal of Experimental Medicine, 216(9), 2038-2056.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!